Anti-JNK1/2 Antibody (E-5) recommended for detection of JNK1 and JNK2 p46 and p54 isoforms of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-JNK1/2 Antibody (E-5): sc-137020

Anti-JNK1/2 Antibody (E-5) is rated 4.7 out of 5 by 6.
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Datasheets

    • JNK1/2 Antibody (E-5) is a mouse monoclonal IgG1 κ, cited in 15 publications, provided at 200 µg/ml
    • raised against amino acids 1-384 representing full length JNK1 p461 p46 of human origin
    • recommended for detection of JNK1 and JNK2 p46 and p54 isoforms of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • See JNK (D-2): sc-7345 for JNK1/2 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for JNK1/2 Antibody (E-5). This work is in progress.
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Can JNK1/2 (E-5): sc-137020 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Cweed
Thank you for your question. Yes, JNK1/2 (E-5): sc-137020 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Great results in WB. Worked well with single strong band showed inHuman pulmonary alveolar epithelial cells.
Date published: 2021-07-09
Rated 5 out of 5 by from Good cytoplasmic staining for IHC with formalin Good cytoplasmic staining for IHC with formalin fixed, paraffin-embedded human rectum tissue. -SCBT QC
Date published: 2015-04-26
Rated 5 out of 5 by from Strong band for WB in TNF treated HeLa (A) Strong band for WB in TNF treated HeLa (A), UV irradiated HeLa (B), UV irradiated NIH/3T3 (C), PC-12 (D) and TNF NIH/3T3 (E) whole cell lysates. -SCBT QC
Date published: 2015-02-10
Rated 3 out of 5 by from Good signal strength with some background Good signal strength with some background for WB in A-431 (A), KNRK (B), NIH/3T3 (C), HeLa (D), K-562 (E), 293T (F) and RAW 264.7 (G) whole cell lysates. -SCBT QC
Date published: 2015-02-04
Rated 5 out of 5 by from Great nuclear staining showed in formalin Great nuclear staining showed in formalin-fixed HepG2 cells by IF. -SCBT QC
Date published: 2015-02-02
Rated 5 out of 5 by from Worked well with human recombinant JNK1 Worked well with human recombinant JNK1, single strong band showed. -SCBT QC
Date published: 2014-07-18
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