Anti-JNK1 Antibody (D-6) recommended for detection of JNK1 p46 and p54 isoforms of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-JNK1 Antibody (D-6): sc-137018

Anti-JNK1 Antibody (D-6) is rated 4.9 out of 5 by 8.
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Datasheets

    • JNK1 Antibody (D-6) is a mouse monoclonal IgG2b κ, cited in 23 publications, provided at 200 µg/ml
    • raised against amino acids 1-384 representing full length JNK1 p461 p46 of human origin
    • Anti-JNK1 Antibody (D-6) is recommended for detection of JNK1 p46 and p54 isoforms of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-JNK1 Antibody (D-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of JNK1 (D-6): sc-137018.
    • m-IgG Fc BP-HRP is the preferred secondary detection reagent for JNK1 Antibody (D-6) for WB and IHC(P) applications. This reagent is now offered in a bundle with JNK1 Antibody (D-6) (see ordering information below).
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What is the species cross-reactivity of this antibody?

Asked by: Zocco
Thank you for your question. This antibody sc-137018 is recommended for detection of JNK1 p46 and p54 isoforms of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. If you are interested in receiving a sample of this antibody to test in a different species, please contact our Technical Service.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-31

I am using JNK1 (D-6): sc-137018 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: DefinitelyNotMatt
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from 很好用,条带清晰 解决了我的燃眉之急,之前买的抗体不够用,又不好意思向老板申请再重新买,于是就和师妹一起申请了试用装,感谢感谢
Date published: 2021-05-31
Rated 4 out of 5 by from 没有孵出图片那么明显的条带 和图片示例的两条带略微有些差异,不知道是否是因为我切割了膜的关系,不过还是可以较为明显的看出来目的条带
Date published: 2017-10-20
Rated 5 out of 5 by from Very good I had tested it in C42B and VCaP cell lines. It works very good.
Date published: 2017-10-11
Rated 5 out of 5 by from great I am so happy with the sensitivity of the antibody.
Date published: 2017-09-28
Rated 5 out of 5 by from Great for IHC(P) The JNK1 Antibody (D-6): sc-137018 works very well for immunohistochemistry with formalin fixed, paraffin-embedded tissue sections, showing staining pattern localized to the cytoplasm and nucleus. This antibody has also been cited multiple times for further verification.
Date published: 2017-01-30
Rated 5 out of 5 by from Robust nuclear and cytoplasmic staining in Robust nuclear and cytoplasmic staining in paraffin-embedded human breast tissue. -SCBT QC
Date published: 2015-04-27
Rated 5 out of 5 by from Great WB data with publication in mouse rat Great WB data with publication in mouse rat and human samples: Rat HCC and McA-RH7777, mouse MEC and Svec4-10EE2, and human Hek 293. -SCBT Publication Review
Date published: 2015-04-09
Rated 5 out of 5 by from Positive WB data in RAW 264 Positive WB data in RAW 264.7 whole cell lysate. -SCBT QC
Date published: 2015-02-16
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