Anti-JMJD2C Antibody (D-4) recommended for detection of JMJD2C of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-JMJD2C Antibody (D-4): sc-515767

Anti-JMJD2C Antibody (D-4) is rated 5.0 out of 5 by 4.
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Datasheets

    • Anti-JMJD2C Antibody (D-4) is a mouse monoclonal IgG2a κ JMJD2C antibody, cited in 2 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 903-926 within an internal region of JMJD2C of human origin
    • Anti-JMJD2C Antibody (D-4) is recommended for detection of JMJD2C of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-JMJD2C Antibody (D-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of JMJD2C (D-4): sc-515767.
    • m-IgG2a BP-HRP is the preferred secondary detection reagent for JMJD2C Antibody (D-4) for WB applications. This reagent is now offered in a bundle with JMJD2C Antibody (D-4) (see ordering information below).
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What dilution are people using this antibody and having success? At 1:500 theres a lot of noise. Thanks, Matt

Asked by: Matt95
Thank you for your question. We used this primary antibody at a dilution of 1:200 for the Western blot data published on our website. Please contact our Technical Service Department for help in troubleshooting the antibody. Please call us toll-free at (800) 457-3801 option 2, or use the live chat on our website.
Answered by: Technical Support
Date published: 2018-11-21

I need to use an antibody to detect the protein in samples of porcine origin. Do you know if JMJD2C (D-4): sc-515767 mouse monoclonal antibody will work?

Asked by: Cweed
Thank you for your inquiry. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity. We are available by phone, e-mail or chat.
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from good for WB I used this antibody for WB in gastric carcinoma cells and it's quite good and specific (as confirmed by RNAi). Conditions: 40 ug of cell lysate in 10% gel. Ab incubation: 1:1000 dilution in 5% milk/PBST ON 4C.
Date published: 2020-04-14
Rated 5 out of 5 by from Excellent in Western Blotting Western Blotting Dilution 1:1000 CFBE cells (humna bronchial) Specific and strong signal
Date published: 2020-03-02
Rated 5 out of 5 by from So good I really like this antibody, it works so well and so specific.
Date published: 2017-09-21
Rated 5 out of 5 by from good antibody this antibody works very well, I use mouse breast cancer tissues and got a nice specific band.
Date published: 2016-12-19
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