Jagged1 Antibody (E-12)recommended for detection of Jagged1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Jagged1 Antibody (E-12): sc-390177

Jagged1 Antibody (E-12) is rated 4.8 out of 5 by 12.
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Datasheets
    • Jagged1 Antibody (E-12) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1110-1218 of Jagged1 of human origin
    • Jagged1 Antibody (E-12) is recommended for detection of Jagged1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including equine
    • Jagged1 Antibody (E-12) is available conjugated to agarose for IP; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Jagged1 (E-12): sc-390177.
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What is the preferred retrieval buffer for this antibody in IHC-P protocol?

Asked by: Souvik
Thank you for your question. We recommend using one of the following methods for antigen unmasking in formalin-fixed and paraffin embedded samples: 1 Heat treatment (recommended method): Place slides in a container and cover with 10 mM sodium citrate buffer, pH 6.0 (sc-294091); or with 50 mM glycine-HCl buffer (sc-29096), pH 3.5, with 0.01% (w/v) EDTA (sc-29092). Heat at 95° C for 5 minutes. Top off with fresh buffer and heat at 95° C for 5 minutes (optimal incubation time may vary for each tissue type). Allow slides to cool in the buffer for approximately 20 minutes. Wash in deionized H2O three times for 2 minutes each. Aspirate excess liquid from slides. 2 Pepsin: Incubate sections for 10–20 minutes in 0.1% Pepsin (sc-476554) in 0.01 N HCl at room temperature. Wash slides several times in deionized H2O. Aspirate excess liquid from slides. 3 Saponin: Incubate sections for 30 minutes in 0.05% Saponin (sc-280079) in deionized H2O at room temperature. Wash at least three times in PBS. Aspirate excess liquid from slides. You can find additional information in our recommended protocol in the 'Resources' section of our website.
Answered by: Tech Support Europe
Date published: 2019-09-05

What is the recommended storage temperature? How long is it stable at that temperature?

Asked by: science_man
Thank you for your question. We recommend storing this antibody at 4°C for long term storage. We guarantee it to be stable at this temperature for 1 year from the date of shipment.
Answered by: Technical Support
Date published: 2019-01-06

The expected size of JAG1 is 150kDa. I am looking in human neurons and see a very nice specific signal by ICC and WB, however, the band is around 60kDa (with nothing at higher molecular weights). Any thoughts on this? Looks great otherwise!

Asked by: Chaz
Thank you for your question. The expected molecular weight of Jagged1 as detected by Jagged1 Antibody (E-12): sc-390177 is indeed 150 kDa. If you are detecting the protein at a different height please contact our technical service directly for assistance.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-29

Does this antibody show any cross reactivity to Jagged-2?

Asked by: John25
Thank you for your question. Our mouse monoclonal Jagged1 Antibody (E-12): sc-390177 is specific for Jagged1 and does not cross-react with Jagged2. The antibody is raised against amino acids 1110-1218 of Jagged1 of human origin which does not share any significant sequence identity with Jagged2.
Answered by: Technical Support
Date published: 2017-02-16

I would like to use sc-390177 for some double staining. Any tips when all I have are mouse monoclonal antibodies?

Asked by: Cweed
We recommend using directly conjugated antibodies. This product, sc-390177, is available conjugated to AF488, AF647, FITC.
Answered by: Technical Support 5
Date published: 2017-01-22
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Rated 5 out of 5 by from Good antibody! 1:200 diluted for wb of rat protein,the band is strong and special
Date published: 2018-03-21
Rated 4 out of 5 by from Jagged1 antibosy is good. I bought the antibody a month ago (free sample) and the WB result is good.
Date published: 2017-12-31
Rated 4 out of 5 by from good for western blot i received the sample antibody for this one and tried western blot on human skin tissue extracts. It shows a good band.
Date published: 2017-07-05
Rated 5 out of 5 by from very nice result WB experiments are very successful, there can always be a clear band, WB effect is very good.
Date published: 2017-05-28
Rated 5 out of 5 by from quality controled antibody We tried this antibody detect this human liver tissue from dilution 1:500, the effect is very good.
Date published: 2017-05-13
Rated 5 out of 5 by from Works great for IF I bought recently and have stained OP-9 (mouse stromal cells) and cholangiocytes for expression of jagged1 -- With a 1:25 concentration shows clear staining of positive controls.
Date published: 2017-04-03
Rated 5 out of 5 by from Excellent Very good for WB with a dilution factor of 1:5,000.
Date published: 2017-02-12
Rated 5 out of 5 by from Superb Just a great antibody for both western blot and IHC applications; much better product than polyclonal alternatives
Date published: 2017-02-05
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