Ivachtin (CAS 745046-84-8)

Ivachtin (2-[4-Methyl-8-(morpholin-4-ylsulfon yl)-1,3-dioxo-1,3-dihydro-2H-pyrrolo[3,4-c]quinolin-2-yl]ethyl acetate) is a potent, cell-permeable, reversible, non-competitive inhibitor of Caspase-3 (IC₅₀ = 23 nM). Also called Caspase-3 Inhibitor VII. It has been shown to have 10-100 fold selectivity over other caspases and higher anti-apoptotic activity than Z-VAD-FMK (sc-3067). Following use of the inhibitor, standard apoptosis assays can then be used to examine treated cells for evidence of apoptosis.
For the fluorometric determination of caspase-3 activity, the following caspase apoptosis detection kit is available from Santa Cruz: Caspase-3 Kit (sc-4263 AK).

References

1. Kravchenko, D.V., et al. 2005. J. Med. Chem. 48: 3680-3683. PMID: 15916416
2. Gauster, M. et al. 2010. Cell Death Differ. 17(2): 336-345. PMID: 19798107