Thank you for your question. Yes, this information is correct, the antibody is raised against a recombinant protein corresponding to amino acids 1-150 of ISG15 of human origin. The exact epitope recognized by this monoclonal antibody has not been determined.
We do recommend blocking in 5% nonfat milk in TBST (0.05% Tween-20) for one hour at room temperature, and incubating the primary antibody at the starting dilution of 1:200 in 5% nonfat milk + TBST for one hour at room temperature, or overnight at 4C.
Date published: 2019-07-03