Anti-ISG15 Antibody (3E5) recommended for detection of ISG15 of human origin by WB and IP

Anti-ISG15 Antibody (3E5): sc-69701

Anti-ISG15 Antibody (3E5) is rated 4.3 out of 5 by 3.
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Datasheets

    • ISG15 Antibody (3E5) is a mouse monoclonal IgG1, cited in 4 publications, provided at 50 µg/0.5 ml
    • raised against a recombinant protein corresponding to amino acids 1-157 of ISG15 of human origin
    • recommended for detection of ISG15 of human origin by WB and IP
    • See ISG15 (F-9): sc-166755 for ISG15 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ISG15 (3E5): sc-69701.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for ISG15 Antibody (3E5). This work is in progress.
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What blocking solution should I use with ISG15 (3E5): sc-69701 monoclonal antibody for Western blot?

Asked by: jenni
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335) Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support 5
Date published: 2017-02-09
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Rated 5 out of 5 by from Great for your specific purpose The antibody to call upon for WB and IP. Will provide your desired pull-down and give you specific WB bands. It has been published with.
Date published: 2017-01-24
Rated 4 out of 5 by from Worked with transfected lysate by WB Worked with transfected lysate by WB. -SCBT QC
Date published: 2015-04-25
Rated 4 out of 5 by from Crisp band seen in WB using HeLa whole cell lysate Crisp band seen in WB using HeLa whole cell lysate. -SCBT QC
Date published: 2013-02-02
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