Anti-IRS-2 Antibody (B-5) is a mouse monoclonal IgG1IRS-2 antibody provided at 200 µg/ml
specific for an epitope mapping between amino acids 1293-1321 of IRS-2 of mouse origin
Anti-IRS-2 Antibody (B-5) is recommended for detection of IRS-2 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine
Anti-IRS-2 Antibody (B-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
blocking peptide, sc-390761 P
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IRS-2 (B-5): sc-390761.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
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Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
IRS-2 Antibody (B-5) is a high quality monoclonal IRS-2 antibody (also designated IRS-2 antibody) suitable for the detection of the IRS-2 protein of mouse, rat and human origin. IRS-2 Antibody (B-5) is available as both the non-conjugated anti-IRS-2 antibody form, as well as multiple conjugated forms of anti-IRS-2 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. IRS-2, originally described as 4PS, acts as a signaling intermediate downstream of the insulin, IGF-1, IL-4, IL-9 and IL-13 receptors. In IRS-2-deficient mice, reduction in total PI 3-kinase activity by 30% and abolition of downstream activation of protein kinase C (PKC) zeta leads to the development of type 2 diabetes. Additionally, reconstitution with retroviral IRS-2 restores IRS-2/PI 3-kinase/PKC zeta signalling as well as glucose uptake. IRS-2 translocates to the nuclei of mouse embryo fibroblasts expressing the insulin-like growth factor 1 receptor. Various mutations in the IGF-IR can result in an abrogation of or decrease in the translocation of IRS proteins to the nucleoli. IRS-2 is responsible for mitogen-activated protein kinase (MAPK) and protein kinase B (PKB) activation by insulin and is the major adapter molecule linking the insulin receptor to this step.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
I would like to use IRS-2 Antibody (B-5): sc-390761 for double staining with another mouse monoclonal primary antibody. Is this possible?
Asked by: Trav11
Thank you for your question. In cases like this, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, sc-390761 is available conjugated to HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Rated 1 out of
IRS-2 Antibody (B-5) used on mouse liver and brainI used this antibody in combination with an anti-mouse Alexa 594 secondary. The signal was non-sepcific and all over like the crests of waves on the surface of an ocean. My student processed cryosections from 4 different mice of different experimental groups so i don't believe it was due to the stickiness of the tissue. We did use H2O2 for 5 min during the block and permeabilization step. This was not needed but was done because we were doing another antibody as well that required it. I don;' believe that this would inhibit SC antibody since it was washed off well and carried out prior to any of the primary or secondary antibody steps.
Date published: 2018-01-31
Rated 3 out of
band detected in huam granulosa cellsI detected IRS2 in human granulosa cells by WB. A specific band was detected but not strong.
Date published: 2017-07-30
Rated 3 out of
A specific band detectedI detected IRS2 in mouse liver sample by WB.
A specific band in exact size was appeared.
Test conditions; 7.5% gel, 30ug protein. 5% milk/TTBS, blocking 1hr RT, primay o/n 1:100, secondary 1h RT
Date published: 2017-07-25
Rated 3 out of
Could not detect a specific band for IDH1The antibody detected a strong band near 60/65-kda, a less strong band below 75-kDa and a weak band around 150-kDa but none of the bands were affected by IRS2 siRNA.
Date published: 2017-05-18
Rated 5 out of
Works in human MSCsI got a sample of this Antibody to try, and it works great.
Date published: 2017-05-17
Rated 5 out of
Good for WB of IRS-2Consistent Western blot results in mouse, rat and human for IRS-2 using the B-5 clone.
Date published: 2017-01-13
Rated 5 out of
Strong signal in HeLa whole cell lysate by WBStrong signal in HeLa whole cell lysate by WB. -SCBT QC
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