IRS-2 Antibody (B-5)recommended for detection of IRS-2 of mouse, rat and human origin by WB, IP, IF and ELISA

IRS-2 Antibody (B-5): sc-390761

IRS-2 Antibody (B-5) is rated 3.6 out of 5 by 7.
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Datasheets

    • IRS-2 Antibody (B-5) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 1293-1321 of IRS-2 of mouse origin
    • IRS-2 Antibody (B-5) is recommended for detection of IRS-2 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine
    • IRS-2 Antibody (B-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-390761 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IRS-2 (B-5): sc-390761.
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I would like to use IRS-2 Antibody (B-5): sc-390761 for double staining with another mouse monoclonal primary antibody. Is this possible?

Asked by: Trav11
Thank you for your question. In cases like this, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, sc-390761 is available conjugated to HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support 5
Date published: 2017-02-04
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Rated 1 out of 5 by from IRS-2 Antibody (B-5) used on mouse liver and brain I used this antibody in combination with an anti-mouse Alexa 594 secondary. The signal was non-sepcific and all over like the crests of waves on the surface of an ocean. My student processed cryosections from 4 different mice of different experimental groups so i don't believe it was due to the stickiness of the tissue. We did use H2O2 for 5 min during the block and permeabilization step. This was not needed but was done because we were doing another antibody as well that required it. I don;' believe that this would inhibit SC antibody since it was washed off well and carried out prior to any of the primary or secondary antibody steps.
Date published: 2018-01-31
Rated 3 out of 5 by from band detected in huam granulosa cells I detected IRS2 in human granulosa cells by WB. A specific band was detected but not strong.
Date published: 2017-07-30
Rated 3 out of 5 by from A specific band detected I detected IRS2 in mouse liver sample by WB. A specific band in exact size was appeared. Test conditions; 7.5% gel, 30ug protein. 5% milk/TTBS, blocking 1hr RT, primay o/n 1:100, secondary 1h RT
Date published: 2017-07-25
Rated 3 out of 5 by from Could not detect a specific band for IDH1 The antibody detected a strong band near 60/65-kda, a less strong band below 75-kDa and a weak band around 150-kDa but none of the bands were affected by IRS2 siRNA.
Date published: 2017-05-18
Rated 5 out of 5 by from Works in human MSCs I got a sample of this Antibody to try, and it works great.
Date published: 2017-05-17
Rated 5 out of 5 by from Good for WB of IRS-2 Consistent Western blot results in mouse, rat and human for IRS-2 using the B-5 clone.
Date published: 2017-01-13
Rated 5 out of 5 by from Strong signal in HeLa whole cell lysate by WB Strong signal in HeLa whole cell lysate by WB. -SCBT QC
Date published: 2013-12-03
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