IRF-3 Antibody (SL-14)recommended for detection of IRF-3 of human origin by WB, IP, IF and IHC(P)

IRF-3 Antibody (SL-14): sc-33642

IRF-3 Antibody (SL-14) is rated 4.0 out of 5 by 4.
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Datasheets

    • IRF-3 Antibody (SL-14) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against recombinant IRF-3 fusion protein corresponding to human IRF-3
    • recommended for detection of IRF-3 of human origin by WB, IP, IF and IHC(P)
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-33642 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See IRF-3 (SL-12): sc-33641 for IRF-3 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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When should the TransCruz reagent sc-33642 X be used instead of the standard form sc-33642?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-25
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Rated 4 out of 5 by from Good antibody for WB in HeLa whole cell lysate Good antibody for WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-05-03
Rated 4 out of 5 by from Good specificity in WB in human 293T whole Good specificity in WB in human 293T whole cell lysates. -SCBT QC
Date published: 2015-03-19
Rated 4 out of 5 by from Worked well in WB in Jurkat Worked well in WB in Jurkat, HL-60 whole cell lysates and U-937 nuclear extract. -SCBT QC
Date published: 2015-03-09
Rated 4 out of 5 by from Strong band in WB in human heart samples Strong band in WB in human heart samples and Primary cultures of neonatal mouse cardiomyocytes. -SCBT Publication Review
Date published: 2013-10-20
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