IRF-3 Antibody (SL-12)recommended for detection of IRF-3 of mouse, rat and human origin by WB, IP, IF and IHC(P)

IRF-3 Antibody (SL-12): sc-33641

IRF-3 Antibody (SL-12) is rated 4.8 out of 5 by 17.
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Datasheets

    • IRF-3 Antibody (SL-12) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against recombinant IRF-3 fusion protein corresponding to human IRF-3 (amino acids 56-427)
    • IRF-3 Antibody (SL-12) is recommended for detection of IRF-3 of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • IRF-3 Antibody (SL-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-33641 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IRF-3 (SL-12): sc-33641.
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You report that sc-33641 detects IRF3 of mouse and human origin, yet all your examples are human. Do you have an example showing positive WB in mouse cells/tissue, with verification it is NOT present in a KO or is induced in a TG?

Asked by: jjmaher
Thank you for your question. We do not have data in mouse cells or tissues at this time. The antibody is recommended for detection of IRF-3 of mouse, rat and human origin based on sequence identity. If the antibody does not work in any of the recommended applications or species, please contact our Technical Service Department and we would be happy to provide you with a complimentary replacement antibody, refund or credit per our product warranty.
Answered by: Technical Support
Date published: 2017-11-22

When should the TransCruz reagent sc-33641 X be used instead of the standard form sc-33641?

Asked by: jenni
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support 5
Date published: 2017-01-20
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Rated 5 out of 5 by from very beautiful WB I get prospective results by using this IRF3 antibody!
Date published: 2017-12-17
Rated 5 out of 5 by from Very strong signal in WB Shows a very strong signal in WB at a dilution of 1:200 (10 ug protein loaded per lane)
Date published: 2017-10-18
Rated 5 out of 5 by from work well we got it several months ago and it works well, we like it !
Date published: 2017-10-11
Rated 5 out of 5 by from IRF3 Works great in Western Blot. Used in 5% milk powder.
Date published: 2017-10-10
Rated 4 out of 5 by from Why I can't see the nuclear translocation of IRF3? I can detect signal. But can't see any nuclear translocation for IRF3 after poly(I:C) stimulation for macrophage. I don't know why, the antibody I used for IF is 1:250.
Date published: 2017-09-19
Rated 4 out of 5 by from 功能出色 该产品是一个很好的产品,首先效果不错,并且条带清晰明了,而且价格相对便宜,是一个不错的选择,推荐使用
Date published: 2017-09-15
Rated 5 out of 5 by from Suitable for Western Blot Works very well in Western Blot done with lysates of primary human fibroblasts.
Date published: 2017-09-07
Rated 5 out of 5 by from good detection on Western Blot using BEAS2B cells This antibody consistently detects IRF3 on western blots of BEAS2B cell protein
Date published: 2017-05-18
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