IRF-1 Antibody (H-8)recommended for detection of IRF-1 of human origin by WB, IP, IF and ELISA

IRF-1 Antibody (H-8): sc-74530

IRF-1 Antibody (H-8) is rated 4.8 out of 5 by 5.
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Datasheets

    • IRF-1 Antibody (H-8) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 121-325 of IRF-1 of human origin
    • IRF-1 Antibody (H-8) is recommended for detection of IRF-1 of human origin by WB, IP, IF and ELISA
    • IRF-1 Antibody (H-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-74530 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IRF-1 (H-8): sc-74530.
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When should the TransCruz reagent sc-74530 X be used instead of the standard form sc-74530?

Asked by: Professor Griffin
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from 抗体不错 使用了试用装,信号比较好,可以得到 清晰的 western blot 结果 。计划订购 更多量的抗体。
Date published: 2017-09-04
Rated 5 out of 5 by from Worked great in Jurkat Cells We used this antibody in a western blot and got positive results at the correct molecular weight in Jurkat cells
Date published: 2017-01-24
Rated 5 out of 5 by from Great antibody for mouse CoIP Great antibody for mouse CoIP, published using RAW264.7 nuclear extracts -SCBT Publication Review
Date published: 2015-03-11
Rated 5 out of 5 by from Great antibody for Western Great antibody for Western, strong band detected in Jurkat and transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-03-08
Rated 4 out of 5 by from Good signal strength with some background Good signal strength with some background bands for WB in Jurkat nuclear extract.-SCBT QC
Date published: 2013-08-22
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