IRF-1 Antibody (E-4)recommended for detection of IRF-1 of mouse, rat and human origin by WB, IP, IF and ELISA

IRF-1 Antibody (E-4): sc-514544

IRF-1 Antibody (E-4) is rated 4.3 out of 5 by 3.
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Datasheets

    • IRF-1 Antibody (E-4) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 307-328 at the C-terminus of IRF-1 of mouse origin
    • IRF-1 Antibody (E-4) is recommended for detection of IRF-1 of mouse, rat and human origin by WB, IP, IF and ELISA
    • IRF-1 Antibody (E-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-514544 X, 200 µg/0.1 ml)
    • blocking peptide, sc-514544 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IRF-1 (E-4): sc-514544.
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I'm looking for an IRF-1 antibody that will react with mouse and/or rat. Am I correct that this is the only one that has been tested on mouse-originated cells and worked? Thank you!

Asked by: IRF1 antibody
Thank you for your question. This antibody is an excellent choice for detection of IRF-1 in mouse, rat and human samples. We also have two other IRF-1 antibodies that are recommended for mouse and rat and have data in mouse cell lines: IRF-1 Antibody (F-2): sc-514505 and IRF-1 Antibody (F-2): sc-514505.
Answered by: Technical Support
Date published: 2019-05-13

What application is the blocking peptide sc-514544 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from 功能不错~ 这次评论晚了抱歉,IRF1曝光出来条带清楚细腻,大概在不到50kd的位置,特异性也好,整体效果不错~有图有真相~!
Date published: 2017-11-08
Rated 5 out of 5 by from great for WB 1:500 dilution in 5% BSA. RT 2h. Faint non-specific bands. Only tested for BV2 cell.
Date published: 2017-10-07
Rated 4 out of 5 by from 功能很出色 Produced beautiful immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells
Date published: 2017-09-22
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