IRF-1 Antibody (20)recommended for detection of IRF-1 of human origin by WB and IP

IRF-1 Antibody (20): sc-135952

IRF-1 Antibody (20) is rated 5.0 out of 5 by 3.
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Datasheets

    • IRF-1 Antibody (20) is a mouse monoclonal IgG1 provided at 50 µg/0.5 ml
    • raised against amino acids 159-279 of IRF-1 of human origin
    • recommended for detection of IRF-1 of human origin by WB and IP
    • See IRF-1 (E-4): sc-514544 for IRF-1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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I am using IRF-1 (20): sc-135952 antibody for immunoprecipitation, how much antibody should I use?

Asked by: cjMara
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Strong signal for WB in Jurkat whole cell Strong signal for WB in Jurkat whole cell lysate and Jurkat and MOLT-4 nuclear. -SCBT QC
Date published: 2015-05-06
Rated 5 out of 5 by from Single band for WB in MOLT Single band for WB in MOLT-3 whole cell lysate. -SCBT QC
Date published: 2014-03-03
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