Anti-IP3R-II Antibody (A-5) recommended for detection of IP3R-II of mouse, rat, human and origin by WB, IP, IF and ELISA

Anti-IP3R-II Antibody (A-5): sc-398434

Anti-IP3R-II Antibody (A-5) is rated 4.8 out of 5 by 10.
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Datasheets

    • IP3R-II Antibody (A-5) is a mouse monoclonal IgG2b κ, cited in 13 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 2666-2695 at the C-terminus of IP3R-II of human origin
    • IP3R-II Antibody (A-5) is recommended for detection of IP3R-II of mouse, rat, human and origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
    • Anti-IP3R-II Antibody (A-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IP3R-II (A-5): sc-398434.
    • m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for IP3R-II Antibody (A-5) for WB applications. These reagents are now offered in bundles with IP3R-II Antibody (A-5) (see ordering information below).
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I need to use an antibody to detect the protein in samples of avian origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 14
Date published: 2017-02-04
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Rated 4 out of 5 by from Worked well! Worked quite well in WT HEK293T cells, which are known to express IP3R2. Used at 1:1000 dilution in 3% BSA in TBS-T (0.1%) for 24hrs at 4C. Loaded 40,80,120ug whole cell lysate per well (extracted with 1% Triton lysis buffer)
Date published: 2021-08-25
Rated 5 out of 5 by from Very nice Ab! 1h blocking in mPBST, IP3RII ab 1:1000 ON at 4°C, secondary ab 1h at RT; nice signal detected at the expected size, no unspecific bands in this region
Date published: 2017-11-27
Rated 5 out of 5 by from Results show is very good 1:200 dilution at WB, the background is always clean and there is a strong and clear band.
Date published: 2017-10-27
Rated 5 out of 5 by from good antibody I got this ab a month ago...it worked well in my cell system (kidney cells)
Date published: 2017-09-16
Rated 4 out of 5 by from hard to get high molecular protein bands With sc-398434 mouse monoclonal antibody we've got good bands
Date published: 2017-03-16
Rated 5 out of 5 by from specific results IP3R-II gives clean and specific results in WB when used detect mouse tissues.
Date published: 2017-03-11
Rated 5 out of 5 by from I am satisfied with the result. I used this antibody in human lung tissue to do Western blot dilution 1:1000, the effect is very good. I am satisfied with the result.
Date published: 2017-02-09
Rated 5 out of 5 by from I'm satisfied with this antibody. I diluted WB with this antibody 1:000. It gives excellent WB signals. I am satisfied with the result.
Date published: 2017-02-08
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