Anti-IP3R-I Antibody (E-8) recommended for detection of IP3R-I of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-IP3R-I Antibody (E-8): sc-271197

Anti-IP3R-I Antibody (E-8) is rated 4.3 out of 5 by 8.
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Datasheets

    • Anti-IP3R-I Antibody (E-8) is a mouse monoclonal IgG1 κ IP3R-I antibody, cited in 11 publications, provided at 200 µg/ml
    • raised against amino acids 1894-1973 mapping within a cytoplasmic domain of IP3R-I of human origin
    • Anti-IP3R-I Antibody (E-8) is recommended for detection of IP3R-I of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-IP3R-I Antibody (E-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IP3R-I (E-8): sc-271197.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for IP3R-I Antibody (E-8) for WB and IHC(P) applications. These reagents are now offered in bundles with IP3R-I Antibody (E-8) (see ordering information below).
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Hello, all that says this antibody works good, may I ask, which secondary are you using to detect it, I tried the m-IgGκ BP-CFL 555 (1:200) and I do not see signal at all, I used cerebellum as positive control, and no signal.

Asked by: GabyCol
Thank you for your question. The recommended secondary to use with this antibody depends on the application you are using it for. Please contact our technical service department for more information and advice on which products would be best for your application.
Answered by: Technical Service 3
Date published: 2020-11-25

使用这种抗体做出来的是总蛋白还是磷酸化得蛋白?

Asked by: huyi
Thanks for your question.IP3R-I (E-8): sc-271197 is raised against amino acids 1894-1973 mapping within a cytoplasmic domain of IP3R-I of human origin.It's recommended for detetion of totoal IP3R-I of mouse, rat and human origin . 感谢你的问题。IP3R-I (E-8): sc-271197抗原是人源 IP3R-I氨基酸序列的1894-1973。推荐检测 IP3R-I总蛋白。
Answered by: Technical Support 9
Date published: 2020-05-25

Does this antibodywork in zebrafish?

Asked by: MAA8
Thank you for your question. This antibody has not been tested for zebrafish use, and is not recommended or warrantied for this use. If you have any further questions, please contact Technical Service.
Answered by: Tech Service
Date published: 2019-08-28

Why does the band appear @ 200 KD while the protein is 300KD?

Asked by: Jeffrey1234
Thank you for your question. The expected molecular weight of the IP3RI monomer is 313 kDa. If the antibody detects its epitope at a different height in your experiments, please contact our technical service directly so we can assist you.
Answered by: SCBT Heidelberg Support
Date published: 2019-03-20

Can I please receive a FREE 10 µg sample of IP3R-I (E-8): sc-271197.

Asked by: lhzalts
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-14

Does i need to use secondary antibody?

Asked by: gpyaa
Thank you for your inquiry.Yes, you need to choose the secondary antibody according to your experiment aims. For more detailed information about this question, please call us toll-free at (800) 457-3801 option 2, e-mail <[email protected]>, or use the live chat on our website.
Answered by: Technical Support 15
Date published: 2017-12-15

Can IP3R-I (E-8): sc-271197 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jerojero
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, IP3R-I (E-8): sc-271197 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2018-05-31
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Rated 4 out of 5 by from Nice band of expected size in HEK293T cell Worked quite well in HEK293T lysates, but this protein is very sensitive on how samples are prepared and transferred (I used a wet transfer approach for this). Used with success at 1:400 and 1:500 dilution in 5% milk in TBST, detected with Donkey anti-mouse HRP secondary.
Date published: 2021-08-24
Rated 4 out of 5 by from Très bon anticorps pour IF Je suis très contente de cet anticorps qui donne un très joli marquage en IF et qui fonctionne bien pour des expériences de PLA
Date published: 2018-02-07
Rated 4 out of 5 by from Reliable antibody producing a clean signal ON incubation of the primary ab (dil. 1:1000) recommended, since signal is not that strong. But there are no unspecific bands in the relevant region and the signal is clear.
Date published: 2017-11-27
Rated 4 out of 5 by from Works nicely I tried this antibody for early zebrafish embryos and it gave nice results
Date published: 2017-10-30
Rated 4 out of 5 by from IFI Immunofluorescence of anti-IP3R. SHSY5Y were then incubated overnight at 4ºC with antibody against IP3R (1:500). We could observed an ER pattern.
Date published: 2017-06-13
Rated 4 out of 5 by from Works well We purchased this antibody and found it works well.
Date published: 2017-04-03
Rated 5 out of 5 by from Great antibody I have been using this total antibody. I use this antibody diluted 1:500 times for Western blot analysis and have a clear and strong signal. Protein samples of human breast cancer cell line. This is a great antibody, it is worth recommending.
Date published: 2017-01-09
Rated 5 out of 5 by from Nice Western Blot data of IP3R Nice Western Blot data of IP3R-I expression in HuT 78 whole cell lysate. -SCBT QC
Date published: 2013-08-06
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