Anti-Integrin β3/ITGB3/CD61 Antibody (Y2/51) recommended for detection of Integrin β3 of mouse, rat and human origin by IF, IHC(P) and FCM

Anti-Integrin β3/ITGB3/CD61 Antibody (Y2/51): sc-20058

Anti-Integrin β3/ITGB3/CD61 Antibody (Y2/51) is rated 5.0 out of 5 by 5.
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Datasheets

    • Integrin β3/ITGB3/CD61 Antibody (Y2/51) is a mouse monoclonal IgG1 κ, cited in 4 publications, provided at 200 µg/ml
    • raised against PHA-stimulated human mononuclear cells
    • Anti-Integrin β3/ITGB3/CD61 Antibody (Y2/51) is recommended for detection of Integrin β3 of mouse, rat and human origin by IF, IHC(P) and FCM
    • Anti-Integrin β3/ITGB3/CD61 Antibody (Y2/51) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Integrin β3/ITGB3/CD61 (Y2/51): sc-20058.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Integrin β3/ITGB3/CD61 Antibody (Y2/51) for IHC(P) applications. These reagents are now offered in bundles with Integrin β3/ITGB3/CD61 Antibody (Y2/51) (see ordering information below).
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Can Integrin β3 (Y2/51): sc-20058 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: AbPolly
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Integrin β3 (Y2/51): sc-20058 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Produced publishable WB, IF, IHC and FCM data This Intergrin beta 3 monoclonal antibody was featured in several publications for the use of WB, IF, IHC and FCM on human samples
Date published: 2017-02-03
Rated 5 out of 5 by from Worked well using feline mature red blood cells Worked well using feline mature red blood cells, platelets and white blood cells in flow cytometry.
Date published: 2015-06-26
Rated 5 out of 5 by from Produced good quality flow cytometry data Produced good quality flow cytometry data in human peripheral blood leukocytes. -SCBT QC
Date published: 2015-03-02
Rated 5 out of 5 by from Stained intact and ruptured ACL Stained intact and ruptured ACL. Publishable results -SCBT Publication Review
Date published: 2015-02-03
Rated 5 out of 5 by from Great ICC results using this antibody with Great ICC results using this antibody with PFA fixed HSC-3 -SCBT Publication Review
Date published: 2009-07-01
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