Anti-Integrin β1/ITGB1 Antibody (A-4) recommended for detection of Integrin β1 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-Integrin β1/ITGB1 Antibody (A-4): sc-374429

Anti-Integrin β1/ITGB1 Antibody (A-4) is rated 4.5 out of 5 by 22.
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Datasheets

    • Integrin β1/ITGB1 Antibody (A-4) is a mouse monoclonal IgG2a κ, cited in 54 publications, provided at 200 µg/ml
    • raised against amino acids 375-480 mapping within an extracellular domain of Integrin β1 of human origin
    • Anti-Integrin β1/ITGB1 Antibody (A-4) is recommended for detection of Integrin β1 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-Integrin β1/ITGB1 Antibody (A-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Integrin β1/ITGB1 (A-4): sc-374429.
    • m-IgG2a BP-HRP is the preferred secondary detection reagent for Integrin β1/ITGB1 Antibody (A-4) for WB applications. This reagent is now offered in a bundle with Integrin β1/ITGB1 Antibody (A-4) (see ordering information below).
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Does this antibody work with reduced samples (WB)?

Asked by: Nsyja
Thank you for your question. Yes, this antibody is recommended for use in Western Blot under reducing conditions.
Answered by: Technical Service
Date published: 2017-04-24

I need to use an antibody to detect the protein in samples of (other species, e.g., bovine, porcine, canine, equine, avian, non-mammalian) origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 2
Date published: 2017-01-12

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 2
Date published: 2017-01-12
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Rated 5 out of 5 by from Very good for MSC Tested in western blot on lysate of MSCs isolated from endometrial decidual tissue. Tests were run at 1:1000 dilution in PBS + 0.05% tween-20 + 1% fat-free milk. Gives two neat bands between 100 and 150 kDa plus a very faint smear below 100 kDa.
Date published: 2021-07-05
Rated 3 out of 5 by from integrin b1 Got sample of this. Western blot performance was average; similar to featured images on website
Date published: 2018-03-05
Rated 4 out of 5 by from Good for WB (rat and human) While previous rabbit antibody from SCBT (discontinued) did not work for human cell lines this antibody seems to detect human INTGb1 as well. In terms of signal strength, I cannot dilute too much (up to 1:300?) though.
Date published: 2018-02-05
Rated 5 out of 5 by from Good Ab! I received 10 ug free sample of this Ab. I used this Ab for WB with DU145 cell lysate, ( dilution 1/500) and I got a single strong band!
Date published: 2018-02-02
Rated 4 out of 5 by from Gutes Ergebnis im Western Blot Zeigt erforderliche Banden im Western Blot. Etwas Hintergrund ist zu sehen, dieser kann aber klar als unspezifisch eingeordnet werden, deswegen ist das kein Problem.
Date published: 2017-11-29
Rated 4 out of 5 by from not bad IF ,1:100,Testis,can be used to mark the SSC membane.
Date published: 2017-10-10
Rated 5 out of 5 by from Great for WB, requires sonication of samples I could detect the bands (precursor and mature) forms of Integrin Beta1 in HT-29 and HeLa cells. Samples need to be sonicated for the Integrin to be detectable
Date published: 2017-09-22
Rated 5 out of 5 by from good features we used this antibody to detect the expression of integrin in glioma cell lysate by western blot
Date published: 2017-08-14
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