Anti-Integrin β1/ITGB1 Antibody (4B7R) recommended for detection of Integrin β1 of mouse, rat and human origin by IF, IHC(P), FCM and ELISA

Anti-Integrin β1/ITGB1 Antibody (4B7R): sc-9970

Anti-Integrin β1/ITGB1 Antibody (4B7R) is rated 4.8 out of 5 by 10.
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Datasheets

    • Integrin β1/ITGB1 Antibody (4B7R) is a mouse monoclonal IgG1 κ, cited in 113 publications, provided at 200 µg/ml
    • raised against full length Integrin β1 of human origin
    • Anti-Integrin β1/ITGB1 Antibody (4B7R) is recommended for detection of Integrin β1 of mouse, rat and human origin by IF, IHC(P), FCM and ELISA
    • Anti-Integrin β1/ITGB1 Antibody (4B7R) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 405 for IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Integrin β1/ITGB1 (4B7R): sc-9970.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Integrin β1/ITGB1 Antibody (4B7R). This work is in progress.
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I used Integrin β1 (4B7R): sc-9970 for IHC(P), but any tissues (including hepatocytes and endothelial cells) were completely negative for this antibody. I use BOND's auto staining machine. Why the antibody was negative for any tissues? How should I do?

Asked by: Dr HI
Thank you for your question. Please contact your local distributor or technical service directly for assistance regarding your Immunohistochemistry results, we are happy to help troubleshoot this with you.
Answered by: Tech Service
Date published: 2019-11-19

I would like to use Integrin β1 Antibody (4B7R): sc-9970 for double staining with another mouse monoclonal primary antibody. Is this possible?

Asked by: Trav11
Thank you for your question. In cases like this, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, sc-9970 is available conjugated to FITC, PE, Alexa Fluor®405, Alexa Fluor® 488 and Alexa Fluor® 647. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support 2
Date published: 2017-01-18
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Rated 4 out of 5 by from Tried for IF Works good for IF on PFA fixed HeLa cells in 1:75 dilution.
Date published: 2018-02-16
Rated 5 out of 5 by from positive staining Very good in IHC against human liver tissue.very nice
Date published: 2017-05-12
Rated 5 out of 5 by from perfect Used at 1:200 in Immunoperoxidase with human liver tissue, staining is bright and specific
Date published: 2017-04-19
Rated 4 out of 5 by from Boa marcação Utilizei este anticorpo para marcação de imunofluorescência, e funcionou bem. Sem precisar de grandes optimizações. Concentração de 1:10
Date published: 2017-03-23
Rated 5 out of 5 by from Great Integrin Antibody Integrin β1 (4B7R) works great for FCM, IHC, and IF. Not recommended for Western blot (WB).
Date published: 2016-12-28
Rated 5 out of 5 by from WB publication using HaCaT samples WB publication using HaCaT samples -SCBT Publication Review
Date published: 2015-04-07
Rated 5 out of 5 by from Immunoperoxidase staining of formalin Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma showing cytoplasmic and membrane localization. -SCBT QC
Date published: 2015-04-07
Rated 5 out of 5 by from IF staining of methanol IF staining of methanol-fixed HUV-EC-C cells showing membrane and cytoplasmic localization. -SCBT QC
Date published: 2015-04-07
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