Anti-Integrin αM/CD11b Antibody (44) recommended for detection of Integrin αM of mouse, rat and human origin by IP, IF, IHC(P) and FCM

Anti-Integrin αM/CD11b Antibody (44): sc-1186

Anti-Integrin αM/CD11b Antibody (44) is rated 4.6 out of 5 by 8.
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Datasheets

    • Integrin αM/CD11b Antibody (44) is a mouse monoclonal IgG1 κ, cited in 28 publications, provided at 200 µg/ml
    • raised against Integrin αM from Rheumatoid synovial cells and monocytes of human origin
    • Anti-Integrin αM/CD11b Antibody (44) is recommended for detection of Integrin αM of mouse, rat and human origin by IP, IF, IHC(P) and FCM
    • Anti-Integrin αM/CD11b Antibody (44) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Integrin αM/CD11b (44): sc-1186.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Integrin αM/CD11b Antibody (44) for IHC(P) applications. These reagents are now offered in bundles with Integrin αM/CD11b Antibody (44) (see ordering information below).
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For the conjugated versions, do you validate that the antibody still works after conjugation reaction (eg, still binds antigen)?

Asked by: jono
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Dallas Tech Service
Date published: 2021-06-18

What is the epitope of this antibody? Does it anti the C-term or N-term of Integrin alpha M, please? Thank you.

Asked by: yang0511
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined.
Answered by: Technical Support
Date published: 2019-06-16

does this antibody block aM expressing cells binding to ligand?

Asked by: texasgirl
Thank you for your question. No, this antibody is not recommended for neutralization studies.
Answered by: Technical Support
Date published: 2019-01-06

Can Integrin αM (44): sc-1186 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Germaine
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Integrin αM (44): sc-1186 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good antibody This antibody worked very well with fixed frozen mouse tissue sections for IF
Date published: 2018-10-03
Rated 5 out of 5 by from Good with IHC Works fine with IHC (pH 9, 1:50) on tonsils (5 µm FFPE).
Date published: 2018-04-12
Rated 5 out of 5 by from Good signal I used it for IF of frozen mouse spinal cord. Good signals. Might needs heat-mediated retrieval.
Date published: 2017-10-10
Rated 4 out of 5 by from Worked well with formalin Worked well with formalin-fixed, paraffin-embedded human lymphoma in IHC -SCBT QC
Date published: 2014-12-09
Rated 4 out of 5 by from Good enrichment when used to IP human PBL Good enrichment when used to IP human PBL before Western detection -SCBT QC
Date published: 2014-11-03
Rated 5 out of 5 by from Great results in IHC/ICC with human glioma Great results in IHC/ICC with human glioma cell lines A172, U343, U373 and T98G -SCBT Publication Review
Date published: 2013-05-21
Rated 5 out of 5 by from FCM with human THP FCM with human THP-1 cells worked well -SCBT Publication Review
Date published: 2013-05-19
Rated 5 out of 5 by from Used in IF with RLMVEC Used in IF with RLMVEC, NIH3T3, Rat leukocyte -SCBT Publication Review
Date published: 2009-08-03
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