Anti-Integrin α5/ITGA5/CD49e Antibody (P1D6) recommended for detection of Integrin α5 of human and primate origin by WB, IP, IF, IHC(P) and FCM

Anti-Integrin α5/ITGA5/CD49e Antibody (P1D6): sc-13547

Anti-Integrin α5/ITGA5/CD49e Antibody (P1D6) is rated 5.0 out of 5 by 4.
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Datasheets

    • Integrin α5/ITGA5/CD49e Antibody (P1D6) is a mouse monoclonal IgG3 κ, cited in 14 publications, provided at 200 µg/ml
    • raised against lymphokine activated killer cells
    • recommended for detection of Integrin α5 of human and primate origin by WB, IP, IF, IHC(P) and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See Integrin α5/ITGA5/CD49e (C-9): sc-376199 for Integrin α5/ITGA5/CD49e antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Integrin α5/ITGA5/CD49e (P1D6): sc-13547.
    • m-IgG3 BP-HRP is the preferred secondary detection reagent for Integrin α5/ITGA5/CD49e Antibody (P1D6) for WB and IHC(P) applications. This reagent is now offered in a bundle with Integrin α5/ITGA5/CD49e Antibody (P1D6) (see ordering information below).
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Can Integrin α5 (P1D6): sc-13547 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Professor Griffin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Integrin α5 (P1D6): sc-13547 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24

Integrin α5 抗体 (P1D6): sc-13547,can this antibody be used for block experiemnt, inhibition the function of Integrin a5?

Asked by: Lemon
Thank you for your question. This mouse monoclonal antibody, Integrin α5 (P1D6): sc-13547, is not recommended for blocking or inhibition studies of the Integrin α5 protein. We have not tested the antibody in this type of biological activity application. And we are not aware of any literature citing use of the antibody in this application. Integrin α5 (P1D6): sc-13547 is currently recommended for detection of Integrin α5 of human and primate origin by WB, IP, IF, IHC(P) and FCM.
Answered by: Technical Support
Date published: 2016-12-01
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Rated 5 out of 5 by from Positive results We had been used a5 antibodies from another source before but noticed the results are not consistent, varying with batches of antibodies. This antibody works great on human kidney tissues. The IHC results look identical as another α5 antibody (MFR5/5H10): sc-23941.
Date published: 2017-06-06
Rated 5 out of 5 by from Excellent! This antibody works great in FCM analysis of human peripheral blood leukocytes.
Date published: 2017-01-19
Rated 5 out of 5 by from Nice membrane and cytoplasmic staining in Nice membrane and cytoplasmic staining in human tissue without any background. -SCBT QC
Date published: 2015-01-23
Rated 5 out of 5 by from Produced good quality flow cytometry data Produced good quality flow cytometry data -SCBT QC
Date published: 2015-01-08
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