Anti-Integrin α5/ITGA5/CD49e Antibody (MFR5/5H10) recommended for detection of Integrin α5 of mouse, rat and human origin by IF and FCM

Anti-Integrin α5/ITGA5/CD49e Antibody (MFR5/5H10): sc-23941

Anti-Integrin α5/ITGA5/CD49e Antibody (MFR5/5H10) is rated 4.3 out of 5 by 4.
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Datasheets

    • Integrin α5/ITGA5/CD49e Antibody (MFR5/5H10) is a rat monoclonal IgG2a, cited in 1 publications, provided at 200 µg/ml
    • raised against activated C57BL/6 mouse spleen cells
    • recommended for detection of Integrin α5 of mouse, rat and human origin by IF and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Integrin α5/ITGA5/CD49e Antibody (MFR5/5H10). This work is in progress.
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How can Integrin α5 (MFR5/5H10): sc-23941 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: cjMara
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to phycoerythrin (sc-23941 PE) or fluoroscein (sc-23941 FITC).
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Positive results We had been used a5 antibodies from another source before but noticed the results are not consistent, varying with batches of antibodies. This antibody works great on human kidney tissues. The IHC results look identical as another α5 antibody (P1D6): sc-13547.
Date published: 2017-06-06
Rated 4 out of 5 by from Immunofluorescence staining of methanol Immunofluorescence staining of methanol-fixed NIH/3T3 cells shows membrane localization. -SCBT QC
Date published: 2015-03-29
Rated 4 out of 5 by from Published human WB results Published human WB results. -SCBT Publication Review
Date published: 2015-02-07
Rated 4 out of 5 by from Positive staining of IL Positive staining of IL-6 stimulated NIH/3T3 cells in FCM. -SCBT QC
Date published: 2013-05-28
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