Anti-Integrin α5/ITGA5/CD49e Antibody (D-9) recommended for detection of Integrin α5 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-Integrin α5/ITGA5/CD49e Antibody (D-9): sc-166666

Anti-Integrin α5/ITGA5/CD49e Antibody (D-9) is rated 3.0 out of 5 by 1.
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Datasheets

    • Integrin α5/ITGA5/CD49e Antibody (D-9) is a mouse monoclonal IgG2a κ, cited in 2 publications, provided at 200 µg/ml
    • raised against amino acids 840-943 of Integrin α5 of human origin
    • recommended for detection of Integrin α5 of mouse, rat and human origin by WB, IP, IF and ELISA
    • See Integrin α5/ITGA5/CD49e (C-9): sc-376199 for Integrin α5/ITGA5/CD49e antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for Integrin α5/ITGA5/CD49e Antibody (D-9) for WB applications. These reagents are now offered in bundles with Integrin α5/ITGA5/CD49e Antibody (D-9) (see ordering information below).
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What is the recommended fixation method for immunofluorescence staining with Integrin α5 (D-9): sc-166666 monoclonal antibody?

Asked by: cjMara
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-28
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Rated 3 out of 5 by from Positive immunofluorescence staining in methanol Positive immunofluorescence staining in methanol-fixed human Integrin 5 transfected HEK 293T cells. -SCBT QC
Date published: 2014-03-29
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