Anti-Integrin α5/ITGA5/CD49e Antibody (C-9) recommended for detection of Integrin α5 heavy chain of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Integrin α5/ITGA5/CD49e Antibody (C-9): sc-376199

Anti-Integrin α5/ITGA5/CD49e Antibody (C-9) is rated 3.3 out of 5 by 7.
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Datasheets

    • Anti-Integrin α5/ITGA5/CD49e Antibody (C-9) is a mouse monoclonal IgG2b κ Integrin α5/ITGA5/CD49e antibody, cited in 13 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 867-894 at the C-terminus of Integrin α5 of human origin
    • Anti-Integrin α5/ITGA5/CD49e Antibody (C-9) is recommended for detection of Integrin α5 heavy chain of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Integrin α5/ITGA5/CD49e Antibody (C-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Integrin α5/ITGA5/CD49e (C-9): sc-376199.
    • m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Integrin α5/ITGA5/CD49e Antibody (C-9) for WB and IHC(P) applications. These reagents are now offered in bundles with Integrin α5/ITGA5/CD49e Antibody (C-9) (see ordering information below).
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What application is the blocking peptide sc-376199 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from No signal We tried this Ab in hela cells but we did not detect any signal
Date published: 2020-12-02
Rated 4 out of 5 by from Works in WB for human cells I tested this antibody for WB in RCS cells (rat chondrosarcoma), SaOS-2 cells and primary human amniocytes. It gives a nice strong band in human cells but nothing in rat cells.
Date published: 2017-10-27
Rated 5 out of 5 by from good features we used this antibody to detect the expression of integrin in glioma cell lysate by western blot
Date published: 2017-08-14
Rated 5 out of 5 by from Works well for WB Works well for WB. Used the antibody at 1:1000 dilution for MDA-MB-231 cell lysate.
Date published: 2017-06-07
Rated 1 out of 5 by from it did not work for WB it did not pick any right band up but one between 75 and 100KDa in the lysates derived from mousdr macrophages
Date published: 2017-05-09
Rated 3 out of 5 by from Positive antigen detection by WB in transfected Positive antigen detection by WB in transfected lysates. -SCBT QC
Date published: 2014-12-27
Rated 4 out of 5 by from Immunoperoxidase staining of formalin fixed Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue shows membrane and cytoplasmic staining of trophoblastic cells with sc-376199. -SCBT QC
Date published: 2014-11-19
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