Anti-Integrin α5/ITGA5/CD49e Antibody (B-4) recommended for detection of Integrin α5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Integrin α5/ITGA5/CD49e Antibody (B-4): sc-166681

Anti-Integrin α5/ITGA5/CD49e Antibody (B-4) is rated 3.0 out of 5 by 1.
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Datasheets

    • Integrin α5/ITGA5/CD49e Antibody (B-4) is a mouse monoclonal IgG1 κ, cited in 3 publications, provided at 200 µg/ml
    • raised against amino acids 840-943 of Integrin α5 of human origin
    • recommended for detection of Integrin α5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • See Integrin α5/ITGA5/CD49e (C-9): sc-376199 for Integrin α5/ITGA5/CD49e antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Integrin α5/ITGA5/CD49e Antibody (B-4) for WB and IHC(P) applications. These reagents are now offered in bundles with Integrin α5/ITGA5/CD49e Antibody (B-4) (see ordering information below).
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Can Integrin α5 (B-4): sc-166681 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Germaine
Thank you for your question. Yes, Integrin α5 (B-4): sc-166681 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 3 out of 5 by from Positive western blot analysis of Integrin Positive western blot analysis of Integrin 5 expression in human Integrin 5 transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-11-04
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