Anti-Integrin α4/ITGA4/CD49d Antibody (C-2) recommended for detection of Integrin α4 of human origin by WB, IP, IF and ELISA

Anti-Integrin α4/ITGA4/CD49d Antibody (C-2): sc-365569

Anti-Integrin α4/ITGA4/CD49d Antibody (C-2) is rated 4.2 out of 5 by 5.
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Datasheets

    • Integrin α4/ITGA4/CD49d Antibody (C-2) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
    • raised against amino acids 796-1005 mapping near the C-terminus of Integrin α4 of human origin
    • Integrin α4/ITGA4/CD49d Antibody (C-2) is recommended for detection of Integrin α4 of human origin by WB, IP, IF and ELISA
    • Anti-Integrin α4/ITGA4/CD49d Antibody (C-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Integrin α4/ITGA4/CD49d (C-2): sc-365569.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Integrin α4/ITGA4/CD49d Antibody (C-2) for WB applications. These reagents are now offered in bundles with Integrin α4/ITGA4/CD49d Antibody (C-2) (see ordering information below).
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What blocking solution should I use with Integrin α4 (C-2): sc-365569 monoclonal antibody for Western blot?

Asked by: Trav11
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-02-28
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Rated 1 out of 5 by from Didnot work for WB did not work for western - samples were human cells in culture
Date published: 2017-11-21
Rated 5 out of 5 by from good feature we used this antibody to detect the expression of integrin in glioma cell lysate by western blot
Date published: 2017-09-25
Rated 5 out of 5 by from Works very well I used this antibody to detect ITGA4 in mouse bone marrow derived macrophages, and it picks up a simple right band!
Date published: 2017-04-26
Rated 5 out of 5 by from Highly potent and fairly specific antibody This monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was very strong detection of the ~150 kDa target protein in a HeLa/Jurkat cell lysate mix, but the target was not detected in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested or in the HeLa/Jurkat cell lysate mix, except for a medium-strong immunoreactive 36 kDa protein found in both mice and humans.
Date published: 2016-11-10
Rated 5 out of 5 by from Western blot analysis shows Integrin 4 expression Western blot analysis shows Integrin 4 expression in Jurkat (A) and MOLT-4 (B) whole cell lysates. -SCBT QC
Date published: 2014-01-27
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