Anti-Integrin α4/ITGA4/CD49d Antibody (A-7) recommended for detection of Integrin α4 of human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Integrin α4/ITGA4/CD49d Antibody (A-7): sc-365209

Anti-Integrin α4/ITGA4/CD49d Antibody (A-7) is rated 5.0 out of 5 by 3.
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Datasheets

    • Integrin α4/ITGA4/CD49d Antibody (A-7) is a mouse monoclonal IgG2b κ, cited in 5 publications, provided at 200 µg/ml
    • raised against amino acids 796-1005 mapping near the C-terminus of Integrin α4 of human origin
    • Integrin α4/ITGA4/CD49d Antibody (A-7) is recommended for detection of Integrin α4 of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Integrin α4/ITGA4/CD49d Antibody (A-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Integrin α4/ITGA4/CD49d (A-7): sc-365209.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Integrin α4/ITGA4/CD49d Antibody (A-7). This work is in progress.
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I am using Integrin α4 (A-7): sc-365209 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jerojero
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Nice antibody for Integrin expression We used this antibody for western blot in order to detect Integrin expression. We got positive bands shown up at the expected molecular weight in Jurkat cells.
Date published: 2017-01-27
Rated 5 out of 5 by from Immunoperoxidase staining of formalin fixed Immunoperoxidase staining of formalin fixed, paraffin-embedded human lymph node tissue shows membrane and cytoplasmic staining of cells in germinal and non-germinal centers. -SCBT QC
Date published: 2014-01-30
Rated 5 out of 5 by from Strong Strong, specific signal in western blot analysis of Integrin 4 expression in Jurkat whole cell lysate. -SCBT QC
Date published: 2013-11-11
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