Anti-Integrin α4/ITGA4/CD49d Antibody (9F10) recommended for detection of Integrin α4 of human origin by IP, IF and FCM

Anti-Integrin α4/ITGA4/CD49d Antibody (9F10): sc-23933

Anti-Integrin α4/ITGA4/CD49d Antibody (9F10) is rated 4.0 out of 5 by 3.
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Datasheets

    • Integrin α4/ITGA4/CD49d Antibody (9F10) is a mouse monoclonal IgG1, cited in 2 publications, provided at 200 µg/ml
    • raised against immunopurified Integrin α4b (VLA-4) of human origin
    • recommended for detection of Integrin α4 of human origin by IP, IF and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Integrin α4/ITGA4/CD49d Antibody (9F10). This work is in progress.
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How can Integrin α4 (9F10): sc-23933 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jenniferc
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to phycoerythrin (sc-23933 PE) or fluoroscein (sc-23933 FITC).
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from Works very nicely for IF on HeLa samples Works very nicely for IF on HeLa samples. -SCBT QC
Date published: 2015-04-07
Rated 4 out of 5 by from Produced good quality flow cytometry data Produced good quality flow cytometry data -SCBT QC
Date published: 2015-04-07
Rated 4 out of 5 by from Satisfactory membrane staining in methanol Satisfactory membrane staining in methanol-fixed Jurkat cells. -SCBT QC
Date published: 2015-01-27
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