Anti-Integrin α3/ITGA3/CD49c Antibody (Ralph 3.2) recommended for detection of Integrin α3 of rat and, to a lesser extent, mouse origin by IP, IF and FCM

Anti-Integrin α3/ITGA3/CD49c Antibody (Ralph 3.2): sc-7019

Anti-Integrin α3/ITGA3/CD49c Antibody (Ralph 3.2) is rated 4.8 out of 5 by 5.
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Datasheets

    • Integrin α3/ITGA3/CD49c Antibody (Ralph 3.2) is a mouse monoclonal IgG1 κ, cited in 29 publications, provided at 200 µg/ml
    • epitope mapping within the extracellular domain of Integrin α3 of rat origin
    • Integrin α3/ITGA3/CD49c Antibody (Ralph 3.2) is recommended for detection of Integrin α3 of rat and, to a lesser extent, mouse origin by IP, IF and FCM
    • Anti-Integrin α3/ITGA3/CD49c Antibody (Ralph 3.2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Integrin α3/ITGA3/CD49c (Ralph 3.2): sc-7019.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Integrin α3/ITGA3/CD49c Antibody (Ralph 3.2) for WB and IHC(P) applications. These reagents are now offered in bundles with Integrin α3/ITGA3/CD49c Antibody (Ralph 3.2) (see ordering information below).
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Can Integrin α3 (Ralph 3.2): sc-7019 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Integrin α3 (Ralph 3.2): sc-7019 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Worked great for Flow Cytometry on 3611 Worked great for Flow Cytometry on 3611-RF cells. -SCBT QC
Date published: 2015-03-18
Rated 5 out of 5 by from Produced good quality flow cytometry data Produced good quality flow cytometry data -SCBT QC
Date published: 2015-02-02
Rated 5 out of 5 by from Mouse ES cell lines (E14Tg2a and B6G Mouse ES cell lines (E14Tg2a and B6G-2) were investigated with this antibody in flow cytometry. Publishable results produced. No significant cross-reactivity with other proteins was observed -SCBT Publication Review
Date published: 2013-06-03
Rated 5 out of 5 by from Publishable data using this antibody (5 Publishable data using this antibody (5 g/mL) for cell attachment assay -SCBT Publication Review
Date published: 2011-01-17
Rated 4 out of 5 by from with 20 g transfected lysate as positive control with 20 g transfected lysate as positive control
Date published: 2000-02-01
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