Anti-Integrin α3/ITGA3/CD49c Antibody (P1B5) recommended for detection of Integrin α3 of human origin by IP, IF, IHC(P) and FCM

Anti-Integrin α3/ITGA3/CD49c Antibody (P1B5): sc-13545

Anti-Integrin α3/ITGA3/CD49c Antibody (P1B5) is rated 5.0 out of 5 by 5.
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Datasheets

    • Integrin α3/ITGA3/CD49c Antibody (P1B5) is a mouse monoclonal IgG1 κ, cited in 29 publications, provided at 200 µg/ml
    • raised against purified glycoprotein-enriched placenta cells of human origin
    • Integrin α3/ITGA3/CD49c Antibody (P1B5) is recommended for detection of Integrin α3 of human origin by IP, IF, IHC(P) and FCM
    • Anti-Integrin α3/ITGA3/CD49c Antibody (P1B5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Integrin α3/ITGA3/CD49c (P1B5): sc-13545.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Integrin α3/ITGA3/CD49c Antibody (P1B5) for IHC(P) applications. These reagents are now offered in bundles with Integrin α3/ITGA3/CD49c Antibody (P1B5) (see ordering information below).
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What negative control should be used when using the conjugated version of Integrin α3 (P1B5): sc-13545 monoclonal antibody in flow cytometry?

Asked by: jenni
Thank you for your inquiry. We recommend using the isotype specific normal mouse IgG conjugated to the same fluorophore. For example, if you are using sc-13545 PE, then you would want to use normal mouse IgG1 PE, sc-2866.
Answered by: Technical Support 13
Date published: 2017-01-20

Is Integrin α3 (P1B5): sc-13545 monoclonal antibody available directly conjugated to Alexa Fluor dyes?

Asked by: jenni
Thank you for your question. All requests for directly conjugated versions of this antibody should be directed to our Special Orders team at [email protected] Alternatively, consider using Integrin α3 (A-3): sc-374242, which is available conjugated to Alexa Fluor® 488 or Alexa Fluor® 647. (https://www.scbt.com/scbt/product/integrin-alpha3-antibody-a-3)
Answered by: Technical Support 2
Date published: 2017-01-16
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Rated 5 out of 5 by from Strong IHC and FCM Very nice product for fcm applications; also strong IHC
Date published: 2017-01-25
Rated 5 out of 5 by from Good FCM antibody Integrin α3 (P1B5) works well for Flow cytometry (FCM).
Date published: 2017-01-18
Rated 5 out of 5 by from Produced good quality flow cytometry data Produced good quality flow cytometry data in human peripheral blood leukocytes. -SCBT QC
Date published: 2015-04-07
Rated 5 out of 5 by from IHC publication with various paraffin IHC publication with various paraffin-embedded human tumors. -SCBT Publication Review
Date published: 2015-03-26
Rated 5 out of 5 by from IHC publication with paraformaldehyde fixed IHC publication with paraformaldehyde fixed human breast cancer. -SCBT Publication Review
Date published: 2015-02-17
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