Anti-Integrin α2/ITGA2/CD49b Antibody (P4B4) recommended for detection of Integrin α2 of human origin by WB, IP and IF

Anti-Integrin α2/ITGA2/CD49b Antibody (P4B4): sc-53506

Anti-Integrin α2/ITGA2/CD49b Antibody (P4B4) is rated 4.0 out of 5 by 2.
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Datasheets

    • Integrin α2/ITGA2/CD49b Antibody (P4B4) is a mouse monoclonal IgG2b κ (kappa light chain) provided at 200 µg/ml
    • raised against HT-1080 fibrosarcoma cells of human origin
    • recommended for detection of Integrin α2 of human origin by WB, IP and IF
    • m-IgGκ BP-HRP is the preferred secondary detection reagent for Integrin α2/ITGA2/CD49b Antibody (P4B4) for WB applications. This reagent is now offered in a bundle with Integrin α2/ITGA2/CD49b Antibody (P4B4) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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What is the recommended fixation method for immunofluorescence staining with Integrin α2 (P4B4): sc-53506 monoclonal antibody?

Asked by: Cweed
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Works well for IF applications Works well for IF applications, membrane staining in MeOH HeLa cells. -SCBT QC
Date published: 2015-04-06
Rated 3 out of 5 by from Worked with human platelet extract and human Worked with human platelet extract and human PBL whole cell lysates under non-reducing conditions by WB with some background -SCBT QC
Date published: 2015-01-30
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