Anti-Integrin α2/ITGA2/CD49b Antibody (P1H5) recommended for detection of Integrin α2 and the integrin α2/β1 complex of human origin by IP, IF and FCM

Anti-Integrin α2/ITGA2/CD49b Antibody (P1H5): sc-13546

Anti-Integrin α2/ITGA2/CD49b Antibody (P1H5) is rated 5.0 out of 5 by 5.
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Datasheets

    • Integrin α2/ITGA2/CD49b Antibody (P1H5) is a mouse monoclonal IgG2b κ, cited in 21 publications, provided at 200 µg/ml
    • raised against HT1080 fibrosarcoma cells of human origin
    • recommended for detection of Integrin α2 and the integrin α2/β1 complex of human origin by IP, IF and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Integrin α2/ITGA2/CD49b Antibody (P1H5). This work is in progress.
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Can Integrin α2 (P1H5): sc-13546 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Germaine
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Integrin α2 (P1H5): sc-13546 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Reliable and Specific Antibody Great antibody for IP and Flow, very specific and reliable antibody.
Date published: 2017-02-03
Rated 5 out of 5 by from Great antibody for flow cytometry Great antibody for flow cytometry, good data with human peripheral blood leukocytes. -SCBT QC
Date published: 2015-04-07
Rated 5 out of 5 by from Works well for Western and IP with human NK cells Works well for Western and IP with human NK cells. -SCBT Publication Review
Date published: 2015-03-31
Rated 5 out of 5 by from FCM: Publication in human ovarian cancer cells FCM: Publication in human ovarian cancer cells. -SCBT Publication Review
Date published: 2015-02-15
Rated 5 out of 5 by from Good antibody for Western Good antibody for Western, publications in ACCS8-11, ACCT, ACCH, Acc-312 and ACC-M. -SCBT Publication Review
Date published: 2015-01-11
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