Anti-Integrin α11/ITGA11 Antibody (F-5) recommended for detection of Integrin α11 of human origin by WB, IP, IF and ELISA

Anti-Integrin α11/ITGA11 Antibody (F-5): sc-390091

Anti-Integrin α11/ITGA11 Antibody (F-5) is rated 3.8 out of 5 by 4.
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Datasheets

    • Anti-Integrin α11/ITGA11 Antibody (F-5) is a mouse monoclonal IgG1 κ Integrin α11/ITGA11 antibody, cited in 2 publications, provided at 200 µg/ml
    • raised against amino acids 913-1151 mapping within an internal region of Integrin α11 of human origin
    • Integrin α11/ITGA11 Antibody (F-5) is recommended for detection of Integrin α11 of human origin by WB, IP, IF and ELISA
    • Anti-Integrin α11/ITGA11 Antibody (F-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Integrin α11/ITGA11 (F-5): sc-390091.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Integrin α11/ITGA11 Antibody (F-5) for WB applications. These reagents are now offered in bundles with Integrin α11/ITGA11 Antibody (F-5) (see ordering information below).
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What is the recommended starting dilution for Western blot for sc-390091: Integrin α11 (F-5) monoclonal antibody?

Asked by: DefinitelyNotMatt
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 3 out of 5 by from No bad. We used the ITGA11 antibody at 1/50 dilution in IF. The antibody detected in fibroblastic cells.
Date published: 2022-04-18
Rated 2 out of 5 by from Didn't work in IHC We have tried a free sample of this antibody on human cryosections with acetone fixation and 1:100 diluition of the antibody. No signal was detected (we used another a11 antibody as positive control). A higher concentration of the antibody/longer incubation time could be considered to get a signal..
Date published: 2017-10-31
Rated 5 out of 5 by from good features we used this antibody to detect the expression of integrin in glioma cell lysate by western blot
Date published: 2017-08-14
Rated 5 out of 5 by from Clean blot with single strong band at expected Clean blot with single strong band at expected size using human uterus tissue extract. -SCBT QC
Date published: 2015-04-06
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