Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Integrin β1/ITGB1 Double Nickase Plasmid (h): sc-400097-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Integrin β1/ITGB1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Integrin β1/ITGB1 Double Nickase Plasmid (h) and Integrin β1/ITGB1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ITGB1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Integrin β1/ITGB1 Antibody (A-4): sc-374429
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Integrin β1/ITGB1 Double Nickase Plasmid (h) | sc-400097-NIC | 20 µg | $410.00 | |||
Integrin β1/ITGB1 Double Nickase Plasmid (h2) | sc-400097-NIC-2 | 20 µg | $410.00 |
ITGB1 encodes integrin β1, a core subunit that heterodimerizes with multiple α integrins to mediate cell–extracellular matrix adhesion and mechanotransduction. Through coupling to focal adhesion kinase (FAK), SRC-family kinases, PI3K–AKT, and MAPK signaling, integrin β1 regulates cytoskeletal organization, polarity, survival, and migration. ITGB1-dependent interactions with laminins, collagens, and fibronectin influence epithelial integrity, immune cell trafficking, and stem cell niche behavior. Dysregulated ITGB1 signaling and trafficking are frequently studied in contexts of tumor invasion and metastasis, fibrosis, and inflammatory tissue remodeling.
Integrin β1/ITGB1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ITGB1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ITGB1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ITGB1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ITGB1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.