Insulin Antibody (2D11-H5)recommended for detection of Insulin of mouse, rat and human origin by IP, IF, IHC(P) and ELISA

Insulin Antibody (2D11-H5): sc-8033

Insulin Antibody (2D11-H5) is rated 4.9 out of 5 by 22.
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Datasheets

    • Insulin Antibody (2D11-H5) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-84 representing full length Insulin of porcine origin
    • Insulin Antibody (2D11-H5) is recommended for detection of Insulin of mouse, rat and human origin by IP, IF, IHC(P) and ELISA; also reactive with additional species, including and bovine and porcine
    • Insulin Antibody (2D11-H5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Insulin (2D11-H5): sc-8033.
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How to dilute this antibody? What volumes can i use to arrive at 1:200?

Asked by: anju2004
Thank you for your question. This antibody is provided as 200µg in 1ml (1000µl), which means you have 1µg of antibody per 5µl of solution. So to make a 1:200 dilution, you need to take 1µg (5µl of solution) into 1ml of your diluent (milk/blocking reagent).
Answered by: Tech Service
Date published: 2019-08-30

what is the volume of primary antibody in the vial? sc-8033 200ug/ml?

Asked by: medha2001
Insulin Antibody (2D11-H5) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg in a total volume of 1 ml PBS with 0.1 % gelatin and <0.1 % sodium azide.
Answered by: Tech Support Europe
Date published: 2019-08-30

Can this antibody be use to IF(frozen section)?

Asked by: Tienfen
Thank you for your question. This primary antibody is recommended and warrantied for IF (immunofluorescent staining of fresh or frozen tissues or cells fixed in methanol or acetone).
Answered by: Technical Support
Date published: 2019-04-22

Want to use antibody to distinguish proinsulin and mature insulin,what products are recommended?

Asked by: tinglan
Thank you for your question. Please contact our Technical Service Department and we can help recommend antibodies for the detection of proinsulin and mature insulin depending on what species and application(s) you need to use the antibodies in.
Answered by: Technical Support
Date published: 2019-03-08

antibody: insulin antibody: prmary host : rabbit reactivity : mouse application : western blot conjugation: unconjugated Can i use this antibody for insulin secretion assay by western.

Asked by: firdos
Thank you for your question. Insulin Antibody (2D11-H5): sc-8033 is a mouse monoclonal antibody (raised in a mouse host). And it is not currently recommended for use in Western blot.
Answered by: Technical Support
Date published: 2018-12-21

Can this antibody be used to detect canine insulin?

Asked by: AbPolly
Thank you for your question. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 10
Date published: 2017-01-11

What is the recommended protocol for using this antibody in IHC(P)?

Asked by: AbPolly
Thank you for your question. For detailed protocol information, please contact our Technical Services Department or refer to our online protocol here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 10
Date published: 2017-01-11
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Rated 5 out of 5 by from GREAT FEATURES. I purchased this antibody two weeks ago. It worked very well on pancreatic section. I will buy this antibody if I need it later. Shirley
Date published: 2019-06-27
Rated 5 out of 5 by from Good Antibody for IHC Good signal on European seabass (Dicentrarchus labrax) fixed in Bouin's solution. Diluition 1:50
Date published: 2019-01-16
Rated 5 out of 5 by from Immunohistochemistry: excellent results It works fine for Langerhans islets mouse and rat.
Date published: 2018-05-31
Rated 5 out of 5 by from Works great! Works in hES cells at 1:50 dilution for immunofluorescence
Date published: 2018-03-01
Rated 5 out of 5 by from Good anitibody for ELISA We got 10 ug sample and used for ELISA. It gave a good result, with 1:300 dilution, and can detect ng/ml level human insulin.
Date published: 2018-02-20
Rated 5 out of 5 by from Easy to use on FFPE Mouse We received this as a sample. Before purchase a customer asked us to get a sample and work the antibody up. This is a straight forward, very easy, great looking antibody. Use a general protocol, sodium citrate pH6.0 dilution 1:100 - 1:250.
Date published: 2018-02-10
Rated 4 out of 5 by from Very good got a free sample and used to perform western blot analysis on mouse hippocampi. The detected band is very clear, and seems specific.
Date published: 2018-02-08
Rated 5 out of 5 by from Works beautifully Works beautifully to detect Insulin in murine tissues as confirmed Insulin KO mouse,1:600 dilution been used ,perfect result.
Date published: 2017-07-23
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