Thank you for your question. It should not be a problem to use mouse serum for blocking on mouse tissue with this ImmunoCruz® rabbit ABC Staining System: sc-2018. The mouse serum is to block non-specific binding of the mouse anti-rabbit IgG secondary antibody. It should not effect the specific binding of the rabbit polyclonal primary antibody. If you experience any problems, we would recommend replacing the mouse serum with our animal-free UltraCruz® Blocking Reagent: sc-516214. A link to this product is below for your reference.
Date published: 2020-12-20