Immobilon®-FL PVDF MembraneFor western blotting using fluorescent, chemifluorescence, chromogenic, chemiluminescence or 125I detection methods. 0.45 µm pore size.

Immobilon®-FL PVDF Membrane

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Datasheets

    • PVDF transfer membrane optimized for fluorescence applications
    • For use in standard Western blotting protocols; does not require special procedures
    • Exhibits low background fluorescence, thus improving sensitivity of all fluorescence detection protocols
    • Recommended for use with both, ImmunoCruz® antibody conjugates and mouse IgG Binding Proteins (m-IgG BP) fluorescing in the near-infrared spectrum, and in the visible spectrum
    • For Western blotting using fluorescence, chemifluorescence, chemiluminescence, chromogenic or 125I detection methods
    • Pore size: 0.45 µm
    • Supplied as a 26.5 cm x 3.75 m roll
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Ordering Information

PRODUCT NAME Catalog # UNIT Price Qty FAVORITES
Immobilon®-FL PVDF Membrane, 1 roll sc-516541 1 roll $500.00

Hi, What is the difference between PVDF with 0.2 um pore size and 0.45? Thanks

Asked by: EH1343
Thank you for your question. Western blotting membranes are microporous substrates to which proteins bind; the dimensions of the pore dictate the size of the protein that can successfully bind to the membrane without passing through. Membranes are available in two pore sizes. You have 0.45-µm pore membranes for most analytical blotting experiments, in which proteins have a molecular weight greater than 20 kD. Or there are the smaller 0.2-µm pore membranes, which are most suitable for low molecular weight proteins (less than 15 kD) that might move through larger pores. A good rule to follow: If detecting a protein loaded at low levels, or quantification is critical, choose the smaller size membrane.
Answered by: Tech Service
Date published: 2019-02-14
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Hi, What is the difference between PVDF with 0.2 um pore size and 0.45? Thanks

Asked by: EH1343
Thank you for your question. Western blotting membranes are microporous substrates to which proteins bind; the dimensions of the pore dictate the size of the protein that can successfully bind to the membrane without passing through. Membranes are available in two pore sizes. You have 0.45-µm pore membranes for most analytical blotting experiments, in which proteins have a molecular weight greater than 20 kD. Or there are the smaller 0.2-µm pore membranes, which are most suitable for low molecular weight proteins (less than 15 kD) that might move through larger pores. A good rule to follow: If detecting a protein loaded at low levels, or quantification is critical, choose the smaller size membrane.
Answered by: Tech Service
Date published: 2019-02-14
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