Anti-IL-1ra Antibody (A-4) recommended for detection of IL-1ra of human origin by WB, IP, IF, IHC(P) and ELISA

Anti-IL-1ra Antibody (A-4): sc-374084

Anti-IL-1ra Antibody (A-4) is rated 5.0 out of 5 by 6.
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Datasheets

    • Anti-IL-1ra Antibody (A-4) is a mouse monoclonal IgG1 (kappa light chain) IL-1ra antibody provided at 200 µg/ml
    • raised against amino acids 26-135 of IL-1ra of human origin
    • IL-1ra Antibody (A-4) is recommended for detection of IL-1ra of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-IL-1ra Antibody (A-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IL-1ra (A-4): sc-374084.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for IL-1ra Antibody (A-4). This work is in progress.
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I am using IL-1ra (A-4): sc-374084 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from pretty good it works well in ICC of HGECs,the result is what we wanna get
Date published: 2017-02-25
Rated 5 out of 5 by from Extremely good antibody IL-1ra Antibody worked well in the human samples. It is being used for blotting or staining.
Date published: 2017-02-09
Rated 5 out of 5 by from IHC-P this antibody is suit to do IHC-P in human derived samples
Date published: 2016-12-12
Rated 5 out of 5 by from Nice cytoplasmic staining of squamous epithelial Nice cytoplasmic staining of squamous epithelial cells of formalin fixed, paraffin-embedded human esophagus tissue showing . - SCBT QC
Date published: 2015-03-17
Rated 5 out of 5 by from Good cytoplasmic and nuclear immunofluorescence Good cytoplasmic and nuclear immunofluorescence staining of formalin-fixed HepG2 cells. - SCBT QC
Date published: 2015-01-04
Rated 5 out of 5 by from Positive Western blot analysis of IL Positive Western blot analysis of IL-1ra expression in A-431 whole cell lysate. - SCBT QC
Date published: 2014-08-19
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