IL-1 beta siRNA and shRNA Plasmids (r) rat IL-1β-specific siRNA, shRNA Plasmid and Lentiviral Particle gene silencers

IL-1β siRNA and shRNA Plasmids (r)

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Datasheets

    • Target species: rat; for corresponding mouse product, see IL-1β siRNA and shRNA Plasmids (m)
    • Gene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in length
    • For independent verification of IL-1β gene silencing results, individual duplex components or plasmids are also available upon request
    • Suitable control antibody: IL-1β Antibody (B122): sc-12742 is recommended as control antibody for monitoring of IL-1β expression knockdown by Western blotting or immunofluorescence
    • For further details describing the si/shRNA Gene Silencers click here
    • Gene silencers are intended for noncommercial and research use only. Click here for details.
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could you please help adjust the protocol for shRNA lentiviral particle use when using primary cells such as neonatal rat ventricular myocytes?

Asked by: Angeliki Asimaki
Thank you for your question. You can find our recommended protocol for shRNA transduction by following this link: https://www.scbt.com/scbt/resources/protocols/shrna-plasmid-dna-mediated-inhibition-of-gene-expression. If the parameters of this protocol do not yield the desired results please contact our technical service directly for assistance.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-29
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