Anti-IL-1 beta Antibody (H-8) recommended for detection of IL-1β of human origin by WB, IP, IF and ELISA

Anti-IL-1β Antibody (H-8): sc-515786

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Datasheets

    • IL-1β Antibody (H-8) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 117-269 of IL-1β of human origin
    • recommended for detection of IL-1β of human origin by WB, IP, IF and ELISA
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for IL-1β Antibody (H-8) for WB applications. These reagents are now offered in bundles with IL-1β Antibody (H-8) (see ordering information below).
loading

How much sample do you recommend loading on the gel for Western blot detection using IL-1β (H-8): sc-515786?

Asked by: jerojero
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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