Anti-IL-1 beta Antibody (E7-2-hIL1β) recommended for detection of IL-1β of human origin by WB, IP, IF and IHC(P)

Anti-IL-1β Antibody (E7-2-hIL1β): sc-32294

Anti-IL-1β Antibody (E7-2-hIL1β) is rated 4.1 out of 5 by 34.
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Datasheets

    • IL-1β Antibody (E7-2-hIL1β) is a mouse monoclonal IgG2b κ, cited in 71 publications, provided at 200 µg/ml
    • raised against recombinant IL-1β of human origin
    • IL-1 beta Antibody (E7-2-hIL1β) is recommended for detection of IL-1β of human origin by WB, IP, IF and IHC(P)
    • Anti-IL-1 beta Antibody (E7-2-hIL1β) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IL-1β (E7-2-hIL1β): sc-32294.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for IL-1β Antibody (E7-2-hIL1β) for WB and IHC(P) applications. These reagents are now offered in bundles with IL-1β Antibody (E7-2-hIL1β) (see ordering information below).
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这个抗体 “IL-1β 抗体 (E7-2-hIL1β): sc-32294”能用于检测猪的IL-1β吗?

Asked by: yingyingying
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

can I use this antibody(sc-32294) in mouse brain tissue for western blot?

Asked by: wcl6231
Thank you for your question. This antibody has not yet been tested in mouse and so that is not a recommended species at this time. It is human reactive only. We are still gathering data to show on our website. Check back for updates!
Answered by: Tech Service
Date published: 2020-01-15

Can IL-1β (E7-2-hILβ): sc-32294 monoclonal antibody be used in WB with zebrafish tissue ? Will there be non-specific staining?

Asked by: havana
Thank you for your question. This antibody has not been tested for use on zebrafish samples, so it is not recommended or warrantied for this use. However we do have a publication with this antibody in fish samples (PMID: 30589112). It is fine for WB applications.
Answered by: Tech Service
Date published: 2019-10-28

Can this il- 1b (sc 32294) detects mature form also..please clear

Asked by: Rahul89
Thank you for your question. Yes, this antibody, IL-1β Antibody (E7-2-hIL1β): sc-32294, is recommended for detection of precursor and mature IL-1beta. This has been confirmed through testing against the purified mature IL-1beta protein. In the cell lysates tested, we have also only observed the precursor form. We recommend you consult some of the product citations using this antibody to see if there is any recommendation for inducing expression of the mature form of the protein.
Answered by: Technical Support
Date published: 2019-07-12

Can IL-1β (E7-2-hILβ): sc-32294 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, IL-1β (E7-2-hILβ): sc-32294 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 2 out of 5 by from hl-60 Because I used rabbit antibody had a good result, so I brought this again, but it didn't work in hl-60 cells.
Date published: 2020-09-19
Rated 1 out of 5 by from inconsistent for western blotting Received a free sample of this antibody to test on AC16 cell lysates. Very high lot to lot variability. One lot showed only the mature band while the other showed only the precursor band for the same cell type. Antibody dilution used was 1:200.
Date published: 2018-04-27
Rated 2 out of 5 by from not work well for IF staining for rat brain tissue formaldehyde fixed rat brain tissue, get signal,but not good
Date published: 2017-10-24
Rated 5 out of 5 by from IHC staining The sample arrived soon and it worked well on the dilution 1:200 in IHC-P
Date published: 2017-10-20
Rated 4 out of 5 by from 此抗体功能很不错 已经购买好几次这个抗体了,一如既往的好用。但是也有点缺点,就是有时候此蛋白的切割产物显示不出来,还是以前兔抗的更好用些。
Date published: 2017-09-21
Rated 5 out of 5 by from IL-1β使用评价 大概是在八月份申请的试用装,进行WB试验还可以,效果挺不错的,符合试验预期的结果,希望贵公司越做越好
Date published: 2017-09-20
Rated 5 out of 5 by from IHC The expression of IL-1 beta protein in the brain of rats with cerebral ischemia was analyzed by IHC,This antibody was very useful and we got expected staining which the signal is lower in treated group.1:800 dilution used.
Date published: 2017-09-16
Rated 5 out of 5 by from Very useful Very useful antibody and it worked great.. Very little background and it is easily got the target band.I suggest use dilution 1:500 to detect this protein.
Date published: 2017-09-02
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