Anti-IL-1 beta Antibody (B122) recommended for detection of IL-1β precursor and mature forms of mouse, rat, human and hamster origin by WB, IP and ELISA

Anti-IL-1β Antibody (B122): sc-12742

Anti-IL-1β Antibody (B122) is rated 4.1 out of 5 by 32.
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Datasheets

    • Anti-IL-1β Antibody (B122) is a Armenian hamster monoclonal IgG IL-1β antibody, cited in 63 publications, provided at 200 µg/ml
    • raised against the mature form of IL-1β of mouse origin
    • Anti-IL-1 beta Antibody (B122) is recommended for detection of IL-1β precursor and mature forms of mouse, rat, human and hamster origin by WB, IP and ELISA
    • Anti-IL-1 beta Antibody (B122) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also provided azide-free for neutralization, sc-12742 L, 200 µg/0.1 ml
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IL-1β (B122): sc-12742.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for IL-1β Antibody (B122). This work is in progress.
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Can I use an anti-mouse IgG secondary antiboby with this antibody made in Hamster?

Asked by: PaGar
Thank you for your question. We do not recommend using an anti-mouse IgG secondary antibody for detection of this primary antibody. IL-1β Antibody (B122) is a Armenian hamster monoclonal IgG, and so you will need to use a secondary antibody that is specific for the detection of Armenian hamster IgG. We recommend using our mouse anti-Armenian hamster IgG-HRP: sc-2789, for example, if you need the secondary antibody conjugated to HRP.
Answered by: Technical Support
Date published: 2020-02-06

Please, kindly what is the peptide sequence for IL-1β Antibody (B122): sc-12742

Asked by: Hossam
Thank you for your question. This Armenian hamster monoclonal antibody, IL-1β (B122): sc-12742, is raised against the mature form of IL-1β of mouse origin. The exact sequence used to develop the antibody is not known.
Answered by: Technical Support
Date published: 2017-10-04

Este anticuerpo monoclonal SC-12742, de hámster se utiliza para detectarlo un secundario antimouse con peroxidasa, o un antihamster con peroxidasa?

Asked by: Sandybell México
El anticuerpo secundario apropiado para este anticuerpo es una IgG anti-hámster tal como sc-2789. The appropriate secondary antibody for this antibody is an anti-hamster IgG such as sc-2789.
Answered by: Tech Service 3
Date published: 2017-04-11

Can IL-1β (B122): sc-12742 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Cweed
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, IL-1β (B122): sc-12742 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Ab works good Ab were tested for direct ELISA to analyze the release level of IL-1beta from RAW 264.7 macrophage cell line. Tested conditions: 1:200 dilution in TBS-T overnight at 4°C and 1 h treatment with horseradish peroxidase-conjugated secondary antibody (1:1000 dilution). Measurements were carried out using TMB as a substrate (100 μl/well), reaction was stopped byaddition of 2N H2S04 (100 μl/well).
Date published: 2021-04-29
Rated 5 out of 5 by from good performance in western blot We are satisfactory with this product for its good performance in WB. It detected both precursor and mature form of IL-1β.
Date published: 2020-09-01
Rated 4 out of 5 by from good antibody antibody was used in 1:500 dilution in mice liver lysates. Results were in general good, although the bands had a little higher molecular weight than pretended.
Date published: 2018-09-05
Rated 4 out of 5 by from 功能良好 申请了试用装,用WB检测了小鼠组织样品。提高样品的上样量可以获得不错结果,但分子量与说明书标注的略有出入。
Date published: 2018-05-12
Rated 5 out of 5 by from It can be detected in LPS plus ATP induced NLRP3 It can be detected in LPS plus ATP induced NLRP3 activation in RAW and THP-1
Date published: 2018-02-05
Rated 5 out of 5 by from WB detection I suggest use 1:400 to 1:800 use 5% BSA dilution,it suitable for WB detect mouse brain tissue lysates.
Date published: 2018-01-27
Rated 3 out of 5 by from 一般般 我是两个月前申请的小样,但是做了几次一般般,后面就没怎么用了,感觉效价不是很高,不建议购买,就这样,凑不够字数了
Date published: 2018-01-05
Rated 2 out of 5 by from IF staining in formaldehyde fixed brain try different dilution, 1:50-1:200. does not work good.
Date published: 2017-10-24
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