Anti-IL-1 beta Antibody (AS57) recommended for detection of IL-1β of human origin by WB and IP

Anti-IL-1β Antibody (AS57): sc-52771

Anti-IL-1β Antibody (AS57) is rated 3.5 out of 5 by 4.
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Datasheets

    • IL-1β Antibody (AS57) is a mouse monoclonal IgG2b, cited in 3 publications, provided at 100 µg/ml
    • raised against recombinant IL-1β of human origin
    • recommended for detection of IL-1β of human origin by WB and IP
    • See IL-1β (E7-2-hIL1β): sc-32294 for IL-1β antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for IL-1β Antibody (AS57). This work is in progress.
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I am using IL-1β (AS57): sc-52771 antibody for immunoprecipitation, how much antibody should I use?

Asked by: AbPolly
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Clear Clear, specific band seen in WB in human biologically active IL-1 fusion protein. -SCBT QC
Date published: 2015-04-11
Rated 3 out of 5 by from Robust double band seen in WB in human IL Robust double band seen in WB in human IL-1 transfected 293 whole cell lysates. -SCBT QC
Date published: 2015-03-16
Rated 3 out of 5 by from Good band in WB in human IL Good band in WB in human IL-1 transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-02-06
Rated 4 out of 5 by from Strong band in WB in human IL Strong band in WB in human IL-1 transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-09-16
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