Anti-IL-1 beta Antibody (5C10) recommended for detection of IL-1β of human origin by WB, IP and IF

Anti-IL-1β Antibody (5C10): sc-52013

Anti-IL-1β Antibody (5C10) is rated 4.3 out of 5 by 3.
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Datasheets

    • IL-1β Antibody (5C10) is a mouse monoclonal IgM, cited in 4 publications, provided at 100 µg/ml
    • raised against recombinant human interleukin-1β
    • recommended for detection of IL-1β of human origin by WB, IP and IF
    • See IL-1β (E7-2-hIL1β): sc-32294 for IL-1β antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for IL-1β Antibody (5C10). This work is in progress.
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I am using IL-1β (5C10): sc-52013 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Cweed
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Works well for WB Works good at a dilution of 1:500 for 25ug of neonatal mouse lung lysate
Date published: 2018-05-07
Rated 5 out of 5 by from Specific signal Western blot analysis of IL Specific signal Western blot analysis of IL-1 expression in SK-N-SH whole cell lysate. - SCBT QC
Date published: 2015-03-27
Rated 4 out of 5 by from Positive Western blot analysis of human recombinant Positive Western blot analysis of human recombinant IL-1b fusion protein. - SCBT QC
Date published: 2013-07-04
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