Anti-IL-1 beta Antibody (11E5) recommended for detection of IL-1β of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-IL-1β Antibody (11E5): sc-52012

Anti-IL-1β Antibody (11E5) is rated 3.5 out of 5 by 14.
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Datasheets

    • IL-1β Antibody (11E5) is a mouse monoclonal IgG2b, cited in 60 publications, provided at 100 µg/ml
    • raised against recombinant human interleukin-1β
    • recommended for detection of IL-1β of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • See IL-1β (E7-2-hIL1β): sc-32294 for IL-1β antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IL-1β (11E5): sc-52012.
    • m-IgG Fc BP-HRP is the preferred secondary detection reagent for IL-1β Antibody (11E5) for WB and IHC(P) applications. This reagent is now offered in a bundle with IL-1β Antibody (11E5) (see ordering information below).
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does this antibody has reactivity towards mature IL1-beta (17Kda)?

Asked by: vijju
Thank you for your question. Yes, this antibody is recommended for detection of precursor and mature IL-1beta.
Answered by: Technical Support
Date published: 2019-07-22

Quali cellule usare come controllo positivo e negativo per questo tipo di anticorpo?

Asked by: Andrea1
Thank you for your question. For IL-1β (11E5) we suggest 293 Lysate: sc-111184, SK-N-SH cell lysate: sc-2410 or BJAB whole cell lysate: sc-2207. If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: SCBT Heidelberg Support
Date published: 2018-12-21

这个抗体检测的活化的白介素吗?

Asked by: wen xin 230111
感谢您的提问。请联系我们的技术支持部门。亚洲技术支持电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”。 Thank you for your inquiry. Please contact our Technical Service Department. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 8
Date published: 2018-12-21

Hi, would it be possible to receive a free sample of the IL-1β (11E5): sc-52012 antibody that I could test on my cells?

Asked by: Tiago
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-14

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 9
Date published: 2017-02-16
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Rated 5 out of 5 by from High quality I am very happy to use this product and it is well worth buying
Date published: 2021-03-10
Rated 1 out of 5 by from western blotting-no mature/cleaved band seen Received a free sample to test on AC16 cell lysates. Antibody dilution used was 1:200. Only the precursor band was visible.
Date published: 2018-04-27
Rated 1 out of 5 by from Too Faint for use Received this as a sample. The signal at the highest dilution, overnight incubation is TOO FAINT to be used. considering this was something we had precious little sample of, I am disappointed.
Date published: 2018-02-26
Rated 3 out of 5 by from Schwaches Ergebniss bei IHC-P Leider auch in hohen Konzentrationen (1:50) keine eindeutig spezifische Färbung auf humanen Lungengewebe.
Date published: 2017-11-17
Rated 5 out of 5 by from nice product very nice antibody, specific band,easy to use 1:1000 dilution works
Date published: 2017-11-16
Rated 1 out of 5 by from Disapointing We got two lots of this antibody. None of them worked by IHC-P even if it was clearly written on the antibody datasheet that it should work.
Date published: 2017-11-16
Rated 5 out of 5 by from perfect IHC This antibody works perfectly for IHC. Staining of hippocampal neurons showed a clear staining which co-localized with gephyrin, 1:200 was used.
Date published: 2017-08-12
Rated 5 out of 5 by from Excellent! This antibody works great in western blot analysis of human IL-1β fusion protein.
Date published: 2017-01-18
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