Anti-IL-1 alpha Antibody (B-7) recommended for detection of IL-1α of human origin by WB, IP, IF and IHC(P)

Anti-IL-1α Antibody (B-7): sc-9983

Anti-IL-1α Antibody (B-7) is rated 4.4 out of 5 by 9.
  • y_2021, m_8, d_2, h_19
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.18
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_9
  • loc_en_US, sid_9983, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 90ms
  • REVIEWS, PRODUCT
loading
5
7
4
1
3
0
2
0
1
1

Datasheets

    • IL-1α Antibody (B-7) is a mouse monoclonal IgG2b κ, cited in 25 publications, provided at 200 µg/ml
    • raised against amino acids 113-271 representing mature IL-1α of human origin
    • IL-1 alpha Antibody (B-7) is recommended for detection of IL-1α of human origin by WB, IP, IF and IHC(P)
    • Anti-IL-1 alpha Antibody (B-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IL-1α (B-7): sc-9983.
    • m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for IL-1α Antibody (B-7) for WB and IHC(P) applications. These reagents are now offered in bundles with IL-1α Antibody (B-7) (see ordering information below).
loading

I am using IL-1α (B-7): sc-9983 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Cweed
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
  • y_2021, m_8, d_1, h_19CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.18
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_en_US, sid_9983, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 141ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Good antibody for WB Strong WB signal obtained using this antibody sample at 1:500 dilultion against THP-1 whole cell lysates. Good and quick service. Thanks.
Date published: 2017-05-19
Rated 4 out of 5 by from good antibody for WB used at 1:1000,bands were detected in 35/17 kDa as figure.
Date published: 2017-04-08
Rated 5 out of 5 by from Good choice for IHC(P) Strong and specific IHC staining on paraffin sections. Great choice if you're working on human samples.
Date published: 2017-01-17
Rated 1 out of 5 by from The product doesn t work in ARPE The product doesn t work in ARPE-19.
Date published: 2015-10-30
Rated 5 out of 5 by from Used on a regular basis and published as well Used on a regular basis and published as well. They are working great for us.
Date published: 2015-05-28
Rated 5 out of 5 by from Good IHC staining in dog renal samples Good IHC staining in dog renal samples. -SCBT Publication Review
Date published: 2015-04-19
Rated 5 out of 5 by from Worked well in IHC in rat periapical tissue Worked well in IHC in rat periapical tissue. -SCBT Publication Review
Date published: 2015-03-17
Rated 5 out of 5 by from Good IHC antibody in human bone from the Good IHC antibody in human bone from the interradicular septum. -SCBT Publication Review
Date published: 2015-02-24
  • y_2021, m_8, d_2, h_19
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.18
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_9
  • loc_en_US, sid_9983, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 6ms
  • REVIEWS, PRODUCT