Anti-IL-1 alpha Antibody (ALF-161) recommended for detection of IL-1α of mouse, rat and human origin by WB, IP, IF, FCM and ELISA

Anti-IL-1α Antibody (ALF-161): sc-12741

Anti-IL-1α Antibody (ALF-161) is rated 4.0 out of 5 by 4.
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Datasheets

    • Anti-IL-1α Antibody (ALF-161) is a Armenian hamster monoclonal IgG IL-1α antibody, cited in 2 publications, provided at 200 µg/ml
    • raised against full length purified recombinant IL-1α of mouse origin
    • Anti-IL-1 alpha Antibody (ALF-161) is recommended for detection of IL-1α of mouse, rat and human origin by WB, IP, IF, FCM and ELISA
    • Anti-IL-1 alpha Antibody (ALF-161) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also provided azide-free for neutralization, sc-12741 L, 200 µg/0.1 ml
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IL-1α (ALF-161): sc-12741.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for IL-1α Antibody (ALF-161). This work is in progress.
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Can IL-1α (ALF-161): sc-12741 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, IL-1α (ALF-161): sc-12741 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Mutant BRAF and MEK Inhibitors Regulate the Tumor Protein lysates were prepared in Laemmli sample buffer, separated by SDS-PAGE, and proteins were transferred to polyvinylidene dif-luoride membranes. Immunoreactivity was detected using HRP-con-jugated secondary antibodies (CalBioTech) and chemiluminescence substrate (Thermo Fisher Scientific) on a Versadoc Imaging Sys-tem (Bio-Rad). Primary antibodies, all from Cell Signaling Technol-ogy unless otherwise stated, were as follows: anti-phospho-ERK1/2 (T202/Y204), anti-ERK2 (Santa Cruz Biotechnology), anti-GSDME (Abcam), anti–cleaved caspase-3, anti-BIM/BOD (Enzo Life Sci-ences), anti-IL1α (Santa Cruz Biotechnology), anti-HMGB1, and anti-GAPDH. Cell supernatants were harvested in the absence of FBS in culture medium to avoid distortion of SDS-PAGE. After centrifu-gation to remove cell debris, cell supernatants were concentrated 10×using Amicon Ultra 10K (Sigma-Aldrich). Concentrates were mixed with Laemmli sample buffer (Bio-Rad) and analyzed via Western blotting. For T-cell culture, concentrates were further washed twice with PBS and once with RPMI-1640 medium by spin, and sterilized by filtration through a 0.2-μm filter. Protein gel staining was per-formed using Coomassie Brilliant Blue R-250.
Date published: 2020-10-27
Rated 3 out of 5 by from need more tests I can not get a clear band in a 1:1000 dilution,I will change its concentration to see whether it can work or not
Date published: 2017-03-02
Rated 5 out of 5 by from IHC-P We have detected positive immunofluorescence signal in human IHC-P sclides
Date published: 2016-12-12
Rated 3 out of 5 by from Good specificity in WB Good specificity in WB. -SCBT QC
Date published: 2013-08-16
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