Anti-IL-1 alpha Antibody (5G3) recommended for detection of IL-1α of human origin by WB, IF and IHC(P)

Anti-IL-1α Antibody (5G3): sc-130325

Anti-IL-1α Antibody (5G3) is rated 4.0 out of 5 by 1.
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Datasheets

    • IL-1α Antibody (5G3) is a mouse monoclonal IgG1, cited in 3 publications, provided at 200 µg/ml
    • raised against recombinant IL-1α of human origin
    • recommended for detection of IL-1α of human origin by WB, IF and IHC(P)
    • See IL-1α (ALF-161): sc-12741 for IL-1α antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for IL-1α Antibody (5G3). This work is in progress.
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Can sc-130325: IL-1α (5G3) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: AbPolly
Thank you for your question. Yes, sc-130325: IL-1α (5G3) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Worked great for WB with human recombinant IL Worked great for WB with human recombinant IL-1 .
Date published: 2014-05-31
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