Anti-IL-1 alpha Antibody (3B3) recommended for detection of IL-1α of human origin by WB, IP, IF and FCM

Anti-IL-1α Antibody (3B3): sc-53350

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Anti-IL-1α Antibody (3B3) is rated 2.0 out of 5 by 1.
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Datasheets

    • IL-1α Antibody (3B3) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against recombinant human Interleukin 1α
    • recommended for detection of IL-1α of human origin by WB, IP, IF and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See IL-1α (ALF-161): sc-12741 for IL-1α antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for IL-1α Antibody (3B3) for WB applications. These reagents are now offered in bundles with IL-1α Antibody (3B3) (see ordering information below).
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How can IL-1α (3B3): sc-53350 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: Germaine
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to phycoerythrin (sc-53350 PE) or fluoroscein (sc-53350 FITC).
Answered by: Technical Support
Date published: 2017-03-01
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