Anti-IL-1 alpha Antibody (3564/3B3-14) recommended for detection of IL-1α of human origin by WB, IP, IF and FCM

Anti-IL-1α Antibody (3564/3B3-14): sc-73494

Anti-IL-1α Antibody (3564/3B3-14) is rated 4.5 out of 5 by 2.
  • y_2021, m_8, d_2, h_19
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.18
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_en_US, sid_73494, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 122ms
  • REVIEWS, PRODUCT
loading
5
1
4
1
3
0
2
0
1
0

Datasheets

    • IL-1α Antibody (3564/3B3-14) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
    • raised against recombinant human IL-1α
    • recommended for detection of IL-1α of human origin by WB, IP, IF and FCM
    • available conjugated to phycoerythrin for IF, IHC(P) and FCM
    • See IL-1α (ALF-161): sc-12741 for IL-1α antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for IL-1α Antibody (3564/3B3-14) for WB applications. These reagents are now offered in bundles with IL-1α Antibody (3564/3B3-14) (see ordering information below).
loading

Does this antibody also recognise the mature, cleaved form?

Asked by: agritsenko
Thank you for your question. Unfortunately we do not have information regarding if it can recognize the cleaved form. It was raised against recombinant IL-1α of human origin but the exact epitope has not been mapped.
Answered by: Tech Service
Date published: 2019-01-22

What is the recommended starting dilution for Western blot for sc-73494: IL-1α (3564/3B3-14) monoclonal antibody?

Asked by: cjMara
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
  • y_2021, m_8, d_2, h_19CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.18
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_en_US, sid_73494, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 119ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Produced publishable WB data This IL-1 alpha Antibody was referenced in the literature for the use of WB on human HT-1080
Date published: 2017-01-26
Rated 4 out of 5 by from Specific band detected by Western blot analysis Specific band detected by Western blot analysis of IL-1a expression in HeLa whole cell lysate. - SCBT QC
Date published: 2015-03-11
  • y_2021, m_8, d_2, h_19
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.18
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_en_US, sid_73494, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 14ms
  • REVIEWS, PRODUCT