Anti-IL-1 alpha Antibody (1F3B3) recommended for detection of IL-1α of human origin by WB, IP and IF

Anti-IL-1α Antibody (1F3B3): sc-32814

Anti-IL-1α Antibody (1F3B3) is rated 4.0 out of 5 by 2.
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Datasheets

    • IL-1α Antibody (1F3B3) is a mouse monoclonal IgG (kappa light chain) provided at 200 µg/ml
    • raised against recombinant human IL-1α
    • recommended for detection of IL-1α of human origin by WB, IP and IF
    • See IL-1α (ALF-161): sc-12741 for IL-1α antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for IL-1α Antibody (1F3B3) for WB applications. These reagents are now offered in bundles with IL-1α Antibody (1F3B3) (see ordering information below).
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What is the recommended starting dilution for Western blot for IL-1α (1F3B3): sc-32814 monoclonal antibody?

Asked by: jerojero
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-32814.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support
Date published: 2017-02-28
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Rated 5 out of 5 by from produced intense staining Good antibody, produced intense staining within minutes.
Date published: 2017-02-10
Rated 3 out of 5 by from Worked well in WB in human recombinant IL Worked well in WB in human recombinant IL-1a fusion protein.
Date published: 2013-01-25
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